Thomas J, Nyberg D
University of Illinois, Department of Biological Sciences, Chicago 60680.
Exp Gerontol. 1988;23(6):501-12. doi: 10.1016/0531-5565(88)90062-9.
Vitamin E added to standard Cerophyl medium at 0.025 mg/ml significantly increased the mean clonal life span of 32 lines of Paramecium tetraurelia from 52.5 days and 187 fissions to 59.9 days and 209 fissions (p less than .05) when compared to unsupplemented, paired controls. The age-specific death rates increased exponentially. Regression analyses found a significantly lower rate of increasing mortality for the supplemented lines compared to controls. Weekly fission rates of supplemented lines declined linearly; more slowly than controls, but not significantly so. A second experiment tested two higher levels of supplemental vitamin E (0.10 and 1.00 mg/ml). Sublines receiving 1.00 mg/ml of supplemental vitamin E had higher rates of mortality and lower fission rates initially, but the mortality rates increased and the fission rates decreased more slowly than in sublines receiving 0.10 mg/ml supplemental vitamin E, resulting in maximum clonal life spans of 141 days and 330 fissions (1.00 mg/ml sublines), compared to 74 days and 271 fissions (0.10 mg/ml sublines). Survivorship of the last individual cells (nondividing) of each clone followed an exponential decline, with a significant increase in mean survival time for supplemented compared to unsupplemented cells (p less than .05).
在标准的 Cerophyl 培养基中添加 0.025 毫克/毫升的维生素 E，与未添加维生素 E 的配对对照组相比，显著提高了 32 株四膜虫的平均克隆寿命，从 52.5 天和 187 次裂变增加到 59.9 天和 209 次裂变（p 小于 0.05）。特定年龄的死亡率呈指数增长。回归分析发现，与对照组相比，添加维生素 E 的品系死亡率增加的速率显著更低。添加维生素 E 的品系每周的裂变率呈线性下降；比对照组慢，但差异不显著。第二个实验测试了两个更高水平的补充维生素 E（0.10 和 1.00 毫克/毫升）。接受 1.00 毫克/毫升补充维生素 E 的亚系最初死亡率更高，裂变率更低，但与接受 0.10 毫克/毫升补充维生素 E 的亚系相比，死亡率增加和裂变率下降的速度更慢，导致最大克隆寿命分别为 141 天和 330 次裂变（1.00 毫克/毫升亚系），而接受 0.10 毫克/毫升补充维生素 E 的亚系为 74 天和 271 次裂变。每个克隆最后一个单细胞（不分裂）的存活率呈指数下降，与未补充维生素 E 的细胞相比，补充维生素 E 的细胞平均存活时间显著增加（p 小于 0.05）。
