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本文引用的文献

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Lipid-coated nano-calcium-phosphate (LNCP) for gene delivery.脂质包覆纳米磷酸钙（LNCP）用于基因传递。
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DC-Chol/DOPE cationic liposomes: a comparative study of the influence factors on plasmid pDNA and siRNA gene delivery.DC-Chol/DOPE 阳离子脂质体：影响质粒 pDNA 和 siRNA 基因转染的因素比较研究。
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Rational design of cationic lipids for siRNA delivery.阳离子脂质体的 siRNA 递呈的合理设计。
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Lipid-like materials for low-dose, in vivo gene silencing.用于低剂量体内基因沉默的类脂材料。
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Selective regulation of aromatase expression for drug discovery.选择性调节芳香酶表达以用于药物发现。
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Endocytosis of FcalphaR is clathrin and dynamin dependent, but its cytoplasmic domain is not required.FcαR 的内吞作用依赖网格蛋白和动力蛋白，但不需要其胞质结构域。
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Liposomes and nanoparticles: nanosized vehicles for drug delivery in cancer.脂质体和纳米粒：用于癌症药物递送的纳米载体。
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Flow cytometry and live confocal analysis for the evaluation of the uptake and intracellular distribution of FITC-ODN into HaCaT cells.采用流式细胞术和实时共聚焦分析评估异硫氰酸荧光素标记的寡脱氧核苷酸（FITC-ODN）进入HaCaT细胞的摄取情况和细胞内分布。
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Mol Pharm. 2009 May-Jun;6(3):686-95. doi: 10.1021/mp900093r.
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Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.带正电荷蛋白质介导的哺乳动物细胞穿透、小干扰RNA转染及DNA转染
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SPAN 体作为小干扰 RNA（siRNA）的递药载体。

SPANosomes as delivery vehicles for small interfering RNA (siRNA).

机构信息

Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA.

出版信息

Mol Pharm. 2012 Feb 6;9(2):201-10. doi: 10.1021/mp200426h. Epub 2011 Dec 29.

DOI:10.1021/mp200426h
PMID:22149175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3273546/
Abstract

Nonionic surfactant vesicles, or SPANosomes (SPs), comprised of cationic lipid and sorbitan monooleate (Span 80) were synthesized and evaluated as small interfering RNA (siRNA) vectors. The SPs had a mean diameter of less than 100 nm and exhibited excellent colloidal stability. The SP/siRNA complexes possessed a slightly positive zeta potential of 12 mV and demonstrated a high siRNA incorporation efficiency of greater than 80%. Cryogenic transmission electron microscopy (cryo-TEM) imaging of the SP/siRNA indicated a predominantly core-shell structure. The SP/siRNA complexes were shown to efficiently and specifically silence expression of both green fluorescent protein (GFP) (66% knockdown) and aromatase (77% knockdown) genes in breast cancer cell lines. In addition, the cellular trafficking pathway of the SP/siRNA was investigated by confocal microscopy using molecular beacons as probes for cytosolic delivery. The results showed efficient endosomal escape and cytosolic delivery of the siRNA cargo following internalization of the SP/siRNA complexes. In conclusion, Span 80 is a potent helper lipid, and the SPs are promising vehicles for siRNA delivery.

摘要

非离子型表面活性剂囊泡，或 SPANosomes（SPs），由阳离子脂质和山梨醇单油酸酯（Span 80）组成，被合成并评估为小干扰 RNA（siRNA）载体。SPs 的平均直径小于 100nm，并表现出极好的胶体稳定性。SP/siRNA 复合物具有略微正的 zeta 电位 12mV，并表现出大于 80%的高 siRNA 掺入效率。SP/siRNA 的低温透射电子显微镜（cryo-TEM）成像表明主要为核壳结构。SP/siRNA 复合物被证明能够有效地和特异性地沉默乳腺癌细胞系中绿色荧光蛋白（GFP）（66%的敲低）和芳香酶（77%的敲低）基因的表达。此外，通过使用分子信标作为胞质递送探针的共聚焦显微镜研究了 SP/siRNA 的细胞内运输途径。结果表明，SP/siRNA 复合物内化后，有效的内涵体逃逸和 siRNA 货物的胞质递送。总之，Span 80 是一种有效的辅助脂质，SPs 是 siRNA 递送的有前途的载体。