Division and Laboratory of Gastroenterology, IRCCS Casa Sollievo della Sofferenza, Research Hospital, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo (FG), Italy.
Cancer Invest. 2012 Feb;30(2):98-105. doi: 10.3109/07357907.2011.640650. Epub 2011 Dec 13.
SIRT1 and the clock genes are involved in carcinogenesis. We evaluated SIRT1 expression in 19 human colorectal cancer (CRC) specimens and clock gene expression in SIRT1-overexpressing CaCo2 and SW480 cells. In CRC, SIRT1 mean expression level was decreased. Compared to CaCo2 cells, SW480 cells displayed lower levels of SIRT1 and PER3 and higher levels of ARNTL1, CLOCK, PER1, PER2, CRY1, TIPIN, and CSNKIE. SIRT1 overexpression induced PER1 upregulation in CaCo2 and downregulation in SW480 cells. SIRT1 expression was heterogeneous in human CRC and in CRC cell lines. These results might have relevant implications for a better understanding of colorectal carcinogenesis.
SIRT1 和时钟基因参与了致癌过程。我们评估了 19 个人结直肠癌细胞（CRC）标本中的 SIRT1 表达水平，以及 SIRT1 过表达的 CaCo2 和 SW480 细胞中的时钟基因表达水平。在 CRC 中，SIRT1 的平均表达水平降低。与 CaCo2 细胞相比，SW480 细胞显示出较低水平的 SIRT1 和 PER3，以及较高水平的 ARNTL1、CLOCK、PER1、PER2、CRY1、TIPIN 和 CSNKIE。SIRT1 过表达诱导 CaCo2 中 PER1 的上调和 SW480 细胞中 PER1 的下调。SIRT1 在人结直肠癌细胞和 CRC 细胞系中的表达存在异质性。这些结果可能对更好地理解结直肠发生具有重要意义。
