Lai Chi-Chih, Lin Pai-Mei, Lin Sheng-Fung, Hsu Cheng-Hsien, Lin Hsin-Ching, Hu Ming-Luen, Hsu Cheng-Ming, Yang Ming-Yu
Division of Laryngology, Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 123 Da-Pi Rd., Niaosong District, 833 Kaohsiung City, Taiwan.
Tumour Biol. 2013 Jun;34(3):1847-54. doi: 10.1007/s13277-013-0726-y. Epub 2013 Mar 12.
Head and neck squamous cell carcinoma (HNSCC) include a group of malignant neoplasms that arise from the upper aerodigestive tract and represent the seventh most common cause of cancer-related death. The overall 5-year survival rates have not significantly improved for decades in spite of the advances in the field of oncology and surgery, encouraging further research on factors that might modify disease prognosis. The silent information regulator (SIR) genes (Sirtuins) play key roles in cellular stress and are associated with aging-related diseases including cancer. Currently, seven human sirtuin (SIRT1-7) genes have been identified, but the roles of SIRT genes in HNSCC are still uncertain. Therefore, in this study, we used real-time quantitative reverse transcription-polymerase chain reaction to investigate the expressions of the seven SIRT genes in human HNSCC tissues to assess the changes in cancerous and noncancerous parts and the correlation with different tumor behaviors. Our results demonstrated that the expression levels of SIRT1, SIRT2, SIRT3, SIRT5, SIRT6, and SIRT7 were significantly downregulated in cancerous tissues compared with noncancerous tissues (all p<0.01). The expression levels of SIRT1, SIRT2, SIRT3, SIRT5, and SIRT7 showed downregulation in advanced stages in respect to early stages (p<0.05). These results indicate that the downregulation of SIRT genes expression may contribute to the development of cancer and trigger the neoplastic disease to more advanced stages. Our study indicates that SIRT genes expression could help in the diagnosis and represent a prognostic biomarker in HNSCC.
头颈部鳞状细胞癌（HNSCC）是一组起源于上呼吸道和消化道的恶性肿瘤，是癌症相关死亡的第七大常见原因。尽管肿瘤学和外科领域取得了进展，但总体5年生存率几十年来并未显著提高，这促使人们进一步研究可能影响疾病预后的因素。沉默信息调节因子（SIR）基因（沉默调节蛋白）在细胞应激中起关键作用，并与包括癌症在内的衰老相关疾病有关。目前，已鉴定出7种人类沉默调节蛋白（SIRT1 - 7）基因，但SIRT基因在HNSCC中的作用仍不确定。因此，在本研究中，我们使用实时定量逆转录 - 聚合酶链反应来研究7种SIRT基因在人HNSCC组织中的表达，以评估癌组织和非癌组织中的变化以及与不同肿瘤行为的相关性。我们的结果表明，与非癌组织相比，癌组织中SIRT1、SIRT2、SIRT3、SIRT5、SIRT6和SIRT7的表达水平显著下调（所有p<0.01）。SIRT1、SIRT2、SIRT3、SIRT5和SIRT7的表达水平在晚期相对于早期呈下调（p<0.05）。这些结果表明，SIRT基因表达的下调可能有助于癌症的发展，并促使肿瘤疾病发展到更晚期阶段。我们的研究表明，SIRT基因表达有助于HNSCC的诊断，并代表一种预后生物标志物。
