Merwyn Samuel, Gopalan Natarajan, Singh Anil Kumar, Rai Ganga Prasad, Agarwal Gauri Shanker
Division of High Containment Facility, Defence Research and Development Establishment, Gwalior, Madhya Pradesh, India.
Hybridoma (Larchmt). 2011 Dec;30(6):519-24. doi: 10.1089/hyb.2011.0061.
Histidine-rich protein-2 (HRPII) secreted by Plasmodium falciparum finds its use as a compelling marker in malaria diagnosis and follow-up. Monoclonal antibodies (MAbs) against P. falciparum HRPII are widely used in antibody-based diagnostic systems to detect HRPII protein in blood of malaria-suspected individuals. In this study, a set of five monoclonal antibodies against recombinant HRPII (rHRPII) were generated and assessed for their potential in diagnostics. Three among the five generated MAbs were of IgG1 isotype and the remaining were of IgM isotype. Probing the MAbs against proved P. falciparum infected serum and pooled control sera by immunoblotting revealed that the MAbs were successful in exposing malarial infection. Collectively, the generated MAbs have the potential to be used in immuno-based diagnostic systems uncovering P. falciparum infections.
恶性疟原虫分泌的富含组氨酸蛋白-2（HRPII）在疟疾诊断和随访中用作引人注目的标志物。针对恶性疟原虫HRPII的单克隆抗体（MAb）广泛用于基于抗体的诊断系统，以检测疑似疟疾个体血液中的HRPII蛋白。在本研究中，制备了一组针对重组HRPII（rHRPII）的五种单克隆抗体，并评估了它们在诊断中的潜力。所产生的五种单克隆抗体中有三种是IgG1同种型，其余为IgM同种型。通过免疫印迹法用已证实的恶性疟原虫感染血清和混合对照血清检测这些单克隆抗体，结果表明这些单克隆抗体成功地检测出疟疾感染。总体而言，所产生的单克隆抗体有潜力用于揭示恶性疟原虫感染的免疫诊断系统。
