Hoffmann Shane G, Stallworth Shawn E, Foran David R
Forensic Science Program, School of Criminal Justice, 560 Baker Hall, Michigan State University, East Lansing, MI 48824, USA.
J Forensic Sci. 2012 May;57(3):602-9. doi: 10.1111/j.1556-4029.2011.01982.x. Epub 2011 Dec 8.
Apprehending those who utilize improvised explosive devices (IEDs) is a national priority owing to their use both domestically and abroad. IEDs are often concealed in bags, boxes, or backpacks to prevent their detection. Given this, the goal of the research presented was to identify IED handlers through postblast DNA recovery from IED containers. Study participants were asked to use backpacks for 11 days, after which they served as containers for pipe bombs. Eleven postdeflagration backpack regions likely to be handled were swabbed and analyzed via mini-short tandem repeats (miniSTRs) and alleles were called blind. An experimental consensus method was examined in which profiles from all regions were considered, to help identify spurious drop-in/out. Results were correct for all loci, except one that remained ambiguous. The results show that recovering DNA from IED containers is a viable approach for aiding in the identification of those who may have been involved in an IED event.
由于简易爆炸装置(IED)在国内外都有使用,抓捕使用这类装置的人是一项国家优先任务。简易爆炸装置常常藏于袋子、箱子或背包中,以防被发现。鉴于此,本研究所呈现的研究目标是通过从简易爆炸装置容器中提取爆炸后DNA来识别简易爆炸装置操作者。研究参与者被要求使用背包11天,之后这些背包用作管状炸弹的容器。对11个爆炸后可能被接触过的背包区域进行擦拭取样,并通过微型短串联重复序列(miniSTRs)进行分析,等位基因分析采用盲法。研究了一种实验性共识方法,即考虑所有区域的图谱,以帮助识别虚假的插入/缺失情况。除了一个位点结果仍不明确外,所有位点的结果都是正确的。结果表明,从简易爆炸装置容器中提取DNA是一种可行的方法,有助于识别可能参与简易爆炸装置事件的人员。