Arber N, Zajicek G
Department of Medicine D, Beilinson Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Liver. 1990 Aug;10(4):205-8. doi: 10.1111/j.1600-0676.1990.tb00460.x.
Thirty male adult rats, weighing 250-300 g, were injected with 0.5 muCi [3H]-thymidine. The rats were killed in groups of five, at the following times: 1 h, 14, 30, 60, 90 and 120 days. The livers were fixed in formalin, embedded in paraffin and cut into 5-microns-thick sections, which were then dipped into liquid emulsion for autoradiography. Bile ducts were evaluated in all portal tracts of a section. Obliquely cut bile ducts were excluded. The number of cells paving the duct lumen was defined as bile duct class. Class is roughly proportional to bile duct size. One hour after labelling, labelling index was 3.39 +/- 0.06% (the second number is one standard error of the mean). With time it declined, reaching 0.22 +/- 0.15 on day 60. Initially labelled cells were found in narrow bile ducts and the mean labelled cell was located at class 7.48 +/- 0.63. With time, labelled cells proceeded into wider bile ducts advancing daily 0.122 +/- 0.025 classes. Intra-hepatic bile duct epithelium renews its cells continuously in the same way as epidermis or gastro-intestinal mucosa do. It consists of two kinetic compartments, a progenitor (P) and a functional (Q) compartment. P feeds Q with cells.
选取30只体重在250 - 300克的成年雄性大鼠，注射0.5微居里的[³H] - 胸腺嘧啶核苷。将大鼠按每组5只进行处死，处死时间如下：1小时、14天、30天、60天、90天和120天。取出肝脏，用福尔马林固定，石蜡包埋，切成5微米厚的切片，然后浸入液体乳剂中进行放射自显影。在切片的所有门管区评估胆管。排除斜切的胆管。将覆盖管腔的细胞数量定义为胆管分级。分级大致与胆管大小成正比。标记后1小时，标记指数为3.39±0.06%（第二个数字为均值的一个标准误）。随着时间推移，标记指数下降，在第60天时降至0.22±0.15。最初标记的细胞见于狭窄胆管，平均标记细胞位于7.48±0.63级。随着时间推移，标记细胞进入更宽的胆管，每天前进0.122±0.025级。肝内胆管上皮细胞以与表皮或胃肠道黏膜相同的方式持续更新其细胞。它由两个动力学区室组成，一个祖细胞（P）区室和一个功能（Q）区室。P区室为Q区室提供细胞。
