用于研究人类着床的体外模型。
An in vitro model for the study of human implantation.
机构信息
Department of Obstetrics and Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA.
出版信息
Am J Reprod Immunol. 2012 Feb;67(2):169-78. doi: 10.1111/j.1600-0897.2011.01095.x. Epub 2011 Dec 12.
PROBLEM
Implantation remains the rate-limiting step for the success of in vitro fertilization. Appropriate models to study the molecular aspects of human implantation are necessary in order to improve fertility.
METHODS
First trimester trophoblast cells are differentiated into blastocyst-like spheroids (BLS) by culturing them in low attachment plates. Immortalized human endometrial stromal cells and epithelial cells (ECC-1) were stably transfected with GFP or tdTomato. Co-culture experiments were monitored using Volocity imaging analysis system.
RESULTS
This method demonstrates attachment and invasion of BLS, formed by trophoblast cells, into stromal cells, but not to uterine epithelial cells.
CONCLUSION
We have developed an in vitro model of uterine implantation. The manipulation of this system allows for dual color monitoring of the cells over time. Additionally, specific compounds can be added to the culture media to test how this may affect implantation and invasion. This model is a helpful tool in understanding the complexity of human implantation.
问题
胚胎着床仍是体外受精成功的限速步骤。为了提高生育能力，有必要建立合适的模型来研究人类着床的分子机制。
方法
通过在低附着板上培养，将早孕滋养层细胞分化为胚泡样球体（BLS）。将永生化的人子宫内膜基质细胞和上皮细胞（ECC-1）稳定转染 GFP 或 tdTomato。使用 Volocity 成像分析系统监测共培养实验。
结果
该方法显示滋养层细胞形成的 BLS 附着并侵入基质细胞，但不侵入子宫内膜上皮细胞。
结论
我们已经建立了一种子宫着床的体外模型。该系统的操作允许随时间对细胞进行双色监测。此外，可以向培养基中添加特定化合物，以测试其对着床和浸润的影响。该模型是理解人类着床复杂性的有用工具。
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