通过酶法葡萄糖补料和增加氧传递的组合，在摇瓶培养中高效生产具有生物活性的重组蛋白。

BioSilta Oy, FI-90014 Oulu, Finland.

Microb Cell Fact. 2011 Dec 12;10:107. doi: 10.1186/1475-2859-10-107.

This report describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved.

本报告描述了一种基于酶的葡萄糖释放系统（EnBase®）和高通气摇瓶（Ultra Yield Flask™）的联合使用。这种组合的优势体现在大肠杆菌中表达的重组醇脱氢酶的活性产率提高了 100 多倍。与 Terrific Broth 和 ZYM-5052 自动诱导培养基相比，EnBase 系统主要通过提高每个细胞的生产力来提高产量。Ultra Yield Flask 增加了 4 倍的氧气转移量，使 EnBase 培养物中的细胞密度更高，但其他测试的培养基则不然，因此，每毫升 EnBase 培养物的产物产率进一步提高。