Hinton J C, Gill D R, Lalo D, Plastow G S, Salmond G P
Department of Biological Sciences, University of Warwick, Coventry, UK.
Mol Microbiol. 1990 Jun;4(6):1029-36. doi: 10.1111/j.1365-2958.1990.tb00675.x.
Polygalacturonase (Peh) and other pectolytic enzymes play a crucial role in the maceration of vegetables by soft rot Erwinia spp. We have sequenced the peh gene of Erwinia carotovora subsp. carotovora, and identified its product as a precursor of molecular weight 42,639, and a mature protein of molecular weight 42,200. A putative KdgR-binding site was identified in the region 5' to the peh gene. The Peh protein showed significant homology with Peh from tomato. In addition, we have found homologies between pectin methylesterase and pectate lyase from Erwinia and their counterparts in tomato. These homologies are described, and their significance discussed.
聚半乳糖醛酸酶（Peh）和其他果胶分解酶在软腐欧文氏菌属细菌对蔬菜的浸解过程中起着关键作用。我们已对胡萝卜软腐欧文氏菌胡萝卜亚种的peh基因进行了测序，并确定其产物为分子量42,639的前体和分子量42,200的成熟蛋白。在peh基因5'端区域鉴定出一个假定的KdgR结合位点。Peh蛋白与来自番茄的Peh具有显著同源性。此外，我们还发现欧文氏菌的果胶甲酯酶和果胶酸裂解酶与其在番茄中的对应物之间存在同源性。本文描述了这些同源性，并讨论了它们的意义。
