Robert L S, Allard S, Gerster J L, Cass L, Simmonds J
Plant Research Centre, Centre Experimental Farm, Ottawa, Ontario, Canada.
Plant Mol Biol. 1993 Dec;23(6):1273-8. doi: 10.1007/BF00042360.
A cDNA clone, Sta 44-4, corresponding to a mRNA highly expressed in Brassica napus cv. Westar stamens, was isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 44-4 is synthesized in pollen beginning at the late uninucleate stage and reaches a maximum in trinucleate microspores. Sta 44-4 displayed significant sequence similarity to known pollen polygalacturonase genes. The B. napus pollen polygalacturonase gene was shown to be part of a small gene family and to display some polymorphism among different cultivars.
通过差异筛选分离并鉴定了一个与甘蓝型油菜品种韦斯塔雄蕊中高表达的mRNA相对应的cDNA克隆Sta 44-4。Northern印迹和原位分析表明，Sta 44-4从单核晚期开始在花粉中合成，并在三核小孢子中达到最大值。Sta 44-4与已知的花粉多聚半乳糖醛酸酶基因显示出显著的序列相似性。甘蓝型油菜花粉多聚半乳糖醛酸酶基因被证明是一个小基因家族的一部分，并且在不同品种之间表现出一些多态性。
