凝血酶可刺激培养的人脐静脉内皮细胞产生免疫反应性内皮素-1。

Thrombin stimulates the production of immunoreactive endothelin-1 in cultured human umbilical vein endothelial cells.

作者信息

Kohno M, Yasunari K, Yokokawa K, Murakawa K, Horio T, Kanayama Y, Fuzisawa M, Inoue T, Takeda T

机构信息

First Department of Internal Medicine, Osaka City University Medical School, Japan.

出版信息

Metabolism. 1990 Oct;39(10):1003-5. doi: 10.1016/0026-0495(90)90157-8.

DOI:10.1016/0026-0495(90)90157-8

PMID:2215247

Abstract

The present study was designed to examine the effect of thrombin on endothelin production by cultured human umbilical vein endothelial cells (HUVEC) by radioimmunoassay. Cultured HUVEC released immunoreactive endothelin-1 (ir-endothelin-1) into the medium in a time-dependent manner. This release was inhibited completely by 10 micrograms/mL cycloheximide and is, therefore, directly related to de novo protein synthesis. Further endothelin release was stimulated by the addition of thrombin to the culture medium. Thrombin-induced ir-endothelin-1 release also was inhibited completely by 10 micrograms/mL cycloheximide. These results suggest that HUVEC in culture produce ir-endothelin-1 slowly, but continuously, and that this production is stimulated by thrombin.

摘要

本研究旨在通过放射免疫分析法检测凝血酶对培养的人脐静脉内皮细胞（HUVEC）产生内皮素的影响。培养的HUVEC以时间依赖性方式将免疫反应性内皮素-1（ir-内皮素-1）释放到培养基中。这种释放被10微克/毫升的环己酰亚胺完全抑制，因此与从头蛋白质合成直接相关。向培养基中添加凝血酶可刺激内皮素进一步释放。凝血酶诱导的ir-内皮素-1释放也被10微克/毫升的环己酰亚胺完全抑制。这些结果表明，培养中的HUVEC缓慢但持续地产生ir-内皮素-1，并且这种产生受到凝血酶的刺激。