School of Resource & Environmental Sciences, Wuhan University, Wuhan 430079, PR China.
Plant Physiol Biochem. 2012 Feb;51:153-8. doi: 10.1016/j.plaphy.2011.10.019. Epub 2011 Nov 7.
Plants have been used to restore vegetation in desert region in Shapotou, where naturally biological soil crusts (BSCs) have formed after planting for several years. However, few works have been done on the allelopathic effects between the plants and soil microalgae in BSCs currently. In this study, we investigated the chemical compositions of volatile oil of Artemisia ordosica and its allelopathic effects on photosynthetic system II (PSII) and antioxidant system of Palmellococcus miniatus, a green algae isolated from BSCs. 37 components, consisted of 17 terpenoids, 14 alcohols, 2 esters, 2 ketones and other 2 components were identified in the volatile oil from A. ordosica by GC-MS analysis. High concentration of volatile oil could significantly inhibit the growth and photosynthetic activity (Fv/Fm), and decreased the photosynthetic parameters by affecting photon absorption, electron transport and the reaction center of PSII of P. miniatus, and also cause the significant increase of superoxide dismutase (SOD; EC 1.15.1.) activity, peroxidase (POD; EC 1.11.1.7) activity, reactive oxygen evolution (ROS) and malondialdehyde (MDA) contents of P. miniatus through the combined effects of components in volatile oil. The results indicated that the emission of volatile oil of A. ordosica could inhibit the growth, photosynthesis of P. miniatus through the oxidative damage, and thus might negatively affect the development of BSCs.
植物已被用于恢复沙坡头沙漠地区的植被，在那里，经过几年的种植后，自然形成了生物土壤结皮（BSC）。然而，目前对于植物和 BSC 中的土壤微藻之间的化感作用，几乎没有研究。在这项研究中，我们研究了来自沙生植物蒙古蒿（Artemisia ordosica）挥发油的化学成分及其对从 BSC 中分离出的绿藻小球藻（Palmellococcus miniatus）的光合作用系统 II（PSII）和抗氧化系统的化感作用。通过 GC-MS 分析，从蒙古蒿挥发油中鉴定出 37 种成分，包括 17 种萜类化合物、14 种醇类、2 种酯类、2 种酮类和其他 2 种成分。高浓度的挥发油可显著抑制小球藻的生长和光合作用活性（Fv/Fm），通过影响光子吸收、电子传递和 PSII 的反应中心，降低小球藻的光合作用参数，同时还会导致超氧化物歧化酶（SOD；EC 1.15.1.）活性、过氧化物酶（POD；EC 1.11.1.7）活性、活性氧（ROS）和丙二醛（MDA）含量的显著增加。结果表明，蒙古蒿挥发油的排放可能通过氧化损伤抑制小球藻的生长和光合作用，从而对 BSC 的发育产生负面影响。
