School of Biological Sciences, Flinders University, Adelaide, South Australia 5001, Australia.
Sci Total Environ. 2012 Jan 1;414:585-91. doi: 10.1016/j.scitotenv.2011.11.044. Epub 2011 Dec 7.
In this study, the impact of bacterial and fungal processes on (14)C-hexadecane mineralisation was investigated in weathered hydrocarbon contaminated soil. The extent of (14)C-hexadecane mineralisation varied depending on the bioremediation strategy employed. Under enhanced natural attenuation conditions, (14)C-hexadecane mineralisation after 98 days was 8.5 ± 3.7% compared to <1.2% without nitrogen and phosphorus additions. (14)C-hexadecane mineralisation was further enhanced through Tween 80 amendments (28.9 ± 2.4%) which also promoted the growth of a Phanerochaete chyrsosporium fungal mat. Although fungal growth in weathered hydrocarbon contaminated soil could be promoted through supplementing additional carbon sources (Tween 80, sawdust, compost, pea straw), fungal (14)C-hexadecane mineralisation was negligible when sodium azide was added to soil microcosms to inhibit bacterial activity. In contrast, when fungal activity was inhibited through nystatin additions, (14)C-hexadecane mineralisation ranged from 6.5 ± 0.2 to 35.8 ± 3.8% after 98 days depending on the supplied amendment. Bacteria inhibition with sodium azide resulted in a reduction in bacterial diversity (33-37%) compared to microcosms supplemented with nystatin or microcosms without inhibitory supplements. However, alkB bacterial groups were undetected in sodium azide supplemented microcosms, highlighting the important role of this bacterial group in (14)C-hexadecane mineralisation.
在这项研究中，研究了风化烃污染土壤中细菌和真菌过程对（14）C-十六烷矿化的影响。（14）C-十六烷矿化的程度取决于所采用的生物修复策略。在强化自然衰减条件下，98 天后（14）C-十六烷矿化率为 8.5±3.7%，而不添加氮、磷时为<1.2%。通过添加吐温 80（28.9±2.4%）进一步促进了（14）C-十六烷矿化，这也促进了 Phanerochaete chyrsosporium 真菌垫的生长。尽管通过补充额外的碳源（吐温 80、木屑、堆肥、豌豆秸秆）可以促进风化烃污染土壤中的真菌生长，但当向土壤微宇宙中添加叠氮化钠以抑制细菌活性时，真菌（14）C-十六烷矿化可以忽略不计。相比之下，当通过添加制霉菌素抑制真菌活性时，98 天后（14）C-十六烷矿化率为 6.5±0.2%至 35.8±3.8%，具体取决于所提供的添加剂。与添加制霉菌素或不添加抑制剂的微宇宙相比，叠氮化钠抑制细菌导致细菌多样性减少（33-37%）。然而，在添加叠氮化钠的微宇宙中未检测到 alkB 细菌群，这突出了该细菌群在（14）C-十六烷矿化中的重要作用。
