Baker IDI Heart and Diabetes Institute, Melbourne, Australia.
Heart Lung Circ. 2012 Mar;21(3):131-42. doi: 10.1016/j.hlc.2011.11.002. Epub 2011 Dec 8.
MicroRNAs (miRNAs) are short non-coding RNA molecules that are approximately 22 nucleotides in length. In the last 10 years, miRNA research and discovery has advanced at a rapid rate. This review provides a brief overview of the discovery and biology of miRNAs, and summarises some of the experimental techniques used for isolation, detection, target prediction, and regulation of miRNAs. We also outline experimental workflows for investigators new to the field, and discuss the diagnostic and therapeutic application of miRNAs.
微小 RNA（miRNA）是长度约为 22 个核苷酸的短非编码 RNA 分子。在过去的 10 年中，miRNA 的研究和发现进展迅速。本综述简要概述了 miRNA 的发现和生物学，并总结了一些用于分离、检测、靶标预测和 miRNA 调控的实验技术。我们还概述了该领域新手的实验工作流程，并讨论了 miRNA 的诊断和治疗应用。
