Charité - University Medicine Berlin, Institute of Physiology, Thielallee 71, D-14195 Berlin, Germany.
Eur J Cell Biol. 2012 Feb;91(2):150-5. doi: 10.1016/j.ejcb.2011.10.004. Epub 2011 Dec 10.
NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial role in myogenesis. Furthermore, inhibition of skNAC expression in myoblasts via specific siRNAs might lead to disturbed incorporation of myosin heavy chain (MyHC) into sarcomeres, however, it has no inhibitory effect on absolute MyHC protein levels. Taken together, our data suggest that skNAC regulates specific aspects of myogenesis.
NAC（新生多肽相关复合物）是由一个α和一个β亚基组成的异二聚体。skNAC（骨骼和心肌特异性αNAC 形式）是αNAC 的一种变体，在肌肉分化和再生中被诱导。我们在这里表明，skNAC 的表达受 p38 MAPK 调控，p38 MAPK 在成肌过程中起着关键作用。此外，通过特异性 siRNA 抑制成肌细胞中的 skNAC 表达可能导致肌球蛋白重链（MyHC）掺入肌节受到干扰，但对绝对 MyHC 蛋白水平没有抑制作用。总之，我们的数据表明 skNAC 调节成肌的特定方面。
