Galantos Genetics GmbH, Johann-Joachim-Becher-Weg 30a, 55128 Mainz, Germany.
Forensic Sci Int Genet. 2012 Jul;6(4):461-5. doi: 10.1016/j.fsigen.2011.10.001. Epub 2011 Dec 11.
A new cell isolation technique linked to the aureka® micromanipulation system (aureka®) was used to pick sperm from mixed samples containing sperm and epithelial cells. Both cell types were stained using the HY-LITER™ high-resolution, fluorescent staining kit. To isolate a single sperm of interest under a fluorescent microscope, a specific microsphere picking technique was used. This sensitive and reliable cell identification and isolation technique enables low-copy-number (LCN) DNA profiling, as few as 20 sperm are sufficient for obtaining a full short tandem repeat (STR) profile without any allelic drop out. The presented protocol covers the whole workflow, from sample staining and cell pick up to STR analysis.
一种新的细胞分离技术与 aureka® 显微操作系统（aureka®）相关联，用于从含有精子和上皮细胞的混合样本中挑选精子。使用 HY-LITER™ 高分辨率荧光染色试剂盒对两种细胞类型进行染色。为了在荧光显微镜下分离出单个感兴趣的精子，使用了一种特定的微球挑选技术。这种敏感可靠的细胞识别和分离技术可实现低拷贝数（LCN）DNA 分析，只需 20 个精子即可获得完整的短串联重复序列（STR）图谱，而不会出现任何等位基因缺失。本方案涵盖了从样品染色到细胞采集再到 STR 分析的整个工作流程。
