Thacker J, Fleck E W, Morris T, Rossiter B J, Morgan T L
MRC Radiobiology Unit, Chilton, Didcot, Oxon, Great Britain.
Mutat Res. 1990 Oct;232(2):163-70. doi: 10.1016/0027-5107(90)90121-j.
DNA was analysed from a large set of hamster hprt gene mutants, some induced by ionising radiations and others occurring naturally, to identify those with large alterations in part of the gene. DNA from these mutants was restricted further with different endonucleases and probed to establish the patterns of restriction fragments remaining. Of 15 mutants characterized, one showed a duplication of part of the 5' end of the gene, and the remainder showed deletions of various sizes. It was possible to approximately locate the breakpoints of the deletions by comparison of fragment patterns to a recently-established map of the hamster gene. The relatively small number of mutants examined precludes rigorous analysis of the distribution of breakpoints in the hprt gene, but taken with other recent studies of deletion mutagenesis it is suggested that non-random induction or selection of this type of mutation may occur.
对大量仓鼠次黄嘌呤磷酸核糖转移酶（hprt）基因突变体的DNA进行了分析，其中一些是由电离辐射诱导产生的，另一些是自然发生的，目的是识别那些基因部分区域有大片段改变的突变体。这些突变体的DNA用不同的核酸内切酶进一步酶切，并进行探针杂交，以确定剩余限制性片段的模式。在已鉴定的15个突变体中，有一个显示基因5'端部分区域发生了重复，其余的则显示出不同大小的缺失。通过将片段模式与最近建立的仓鼠基因图谱进行比较，可以大致定位缺失的断点。所检测的突变体数量相对较少，无法对hprt基因中断点的分布进行严格分析，但结合最近其他关于缺失诱变的研究表明，可能会发生这种类型突变的非随机诱导或选择。
