Yandell M D, Edgar L G, Wood W B
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1381-5. doi: 10.1073/pnas.91.4.1381.
To examine the mutagenic spectrum of 4,5',8-trimethylpsoralen (TMP) in Caenorhabditis elegans, we isolated mutations in the unc-22 and pal-1 genes following TMP mutagenesis and analyzed them for restriction fragment length polymorphisms by Southern blot. Eleven of 21 unc-22 mutations exhibited restriction fragment length polymorphisms, 8 of which were deletions of between 0.10 and 15 kb in length. Both of two pal-1 mutations were also small deletions within this size range. Comparison of our results with previous studies on mutagenesis by gamma-rays and x-rays suggests that the mutagenic spectrum of TMP may be similar. TMP should be useful in generating mutations that cause complete loss of function of single genes and that are likely to result in allele-specific DNA polymorphisms.
为了研究4,5',8-三甲基补骨脂素（TMP）在秀丽隐杆线虫中的诱变谱，我们在TMP诱变后分离了unc-22和pal-1基因中的突变，并通过Southern印迹分析它们的限制性片段长度多态性。21个unc-22突变中有11个表现出限制性片段长度多态性，其中8个是长度在0.10至15 kb之间的缺失。两个pal-1突变也都是这个大小范围内的小缺失。将我们的结果与先前关于γ射线和X射线诱变的研究进行比较表明，TMP的诱变谱可能相似。TMP在产生导致单个基因功能完全丧失且可能导致等位基因特异性DNA多态性的突变方面应该是有用的。
