Department of Pharmacy, Saurashtra University, Rajkot, India.
Gut Microbes. 2011 Nov-Dec;2(6):319-25. doi: 10.4161/gmic.18755. Epub 2011 Nov 1.
The protective effect of Lactobacillus rhamnosus 231 (Lr 231) against potent carcinogen N-Methyl-N'-Nitro-N-Nitrosoguanidine (MNNG) in the rat model is studied. Daily feeding with Lr 231 improved the body weight of male Wistar rats compared with control groups. Fecal azoreductase (p < 0.001) and nitroreductase (p < 0.01) enzyme activity decreased significantly in Lr 231 group in comparison with control groups that received only phosphate buffer or MNNG. Oral administration of MNNG led to a significant increase in Glutathione transferase (GST) while Glutathione reductase (GSH) showed decreased activity. Conversely, feeding Lr 231 showed significantly increased GSH and decreased GST activity in comparison to the MNNG group, emphasizing the protection provided by Lr 231 against MNNG. Histopathological analysis of liver, spleen and colon showed decreased signs of inflammation in the Lr 231 group. The present study highlights that inclusion of active Lr 231 in regular diets could be used to prevent MNNG induced colon carcinoma.
研究了鼠李糖乳杆菌 231（Lr 231）对强致癌剂 N-甲基-N'-硝基-N-亚硝基胍（MNNG）在大鼠模型中的保护作用。与对照组相比，每日用 Lr 231 喂养雄性 Wistar 大鼠可改善其体重。与仅接受磷酸盐缓冲液或 MNNG 的对照组相比，Lr 231 组粪便偶氮还原酶（p < 0.001）和硝基还原酶（p < 0.01）酶活性显著降低。口服 MNNG 导致谷胱甘肽转移酶（GST）显著增加，而谷胱甘肽还原酶（GSH）活性降低。相反，与 MNNG 组相比，Lr 231 喂养显示出 GSH 活性显著增加和 GST 活性降低，这强调了 Lr 231 对 MNNG 的保护作用。肝、脾和结肠的组织病理学分析显示 Lr 231 组炎症迹象减少。本研究强调，在常规饮食中加入活性 Lr 231 可能用于预防 MNNG 诱导的结肠癌。
