Savettieri G, Guarneri R, Salemi G, La Bella V, Ferraro D, Scondotto S, Piccoli F
Istituto di Neuropsichiatria, Università degli Studi di Palermo, Italy.
Neurochem Res. 1990 Aug;15(8):773-6. doi: 10.1007/BF00968553.
[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of 3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to 3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected.
体外研究了[3H]氟硝西泮（FNZ）与胎鼠脑皮质神经元的结合。使用对神经元特异的合成培养基，得以在几乎纯的神经元培养物中绘制3H-FNZ结合的发育曲线。在时间0（即妊娠第16天）时体外可检测到特异性结合。在随后的日子里，由于识别位点数量的增加，观察到结合逐渐增加。添加外源性γ-氨基丁酸（GABA）可增强特异性结合位点对3H-FNZ的亲和力，而密度不受影响。
