Cestelli A, Savettieri G, Ferraro D, Vitale F
Brain Res. 1985 Oct;354(2):219-27. doi: 10.1016/0165-3806(85)90173-7.
Dissociated cells from rat fetal cerebral hemispheres were grown in surface adhering culture using a novel synthetic medium (Maat medium) and compared with those grown either in the presence of serum or in the chemically defined medium described by Bottenstein and Sato. The addition of various compound combinations allowed us to lower insulin concentration to almost physiological levels. Maat medium improved the purity and longevity of neuronal cultures. The purity of neuronal cultures grown in different media was checked both by immunofluorescence and by the analysis of [3H]thymidine incorporation.
使用一种新型合成培养基（Maat培养基）在表面贴壁培养中培养大鼠胎儿脑半球的解离细胞，并将其与在血清存在下或在Bottenstein和Sato描述的化学成分确定的培养基中培养的细胞进行比较。添加各种化合物组合使我们能够将胰岛素浓度降低到几乎生理水平。Maat培养基提高了神经元培养物的纯度和寿命。通过免疫荧光和[3H]胸腺嘧啶核苷掺入分析检查在不同培养基中生长的神经元培养物的纯度。
