Edwards M A, Yamamoto M, Caviness V S
Developmental Neurobiology Department, E.K. Shriver Center, Waltham, MA 02254.
Neuroscience. 1990;36(1):121-44. doi: 10.1016/0306-4522(90)90356-9.
A monoclonal antibody, RC1, has been generated which provides a selective and sensitive immunohistochemical marker of radial glial cells and related cell forms during development of the mouse CNS. Beginning on embryonic day E10, immunocytochemistry performed on cryostat sections stains throughout the CNS a subpopulation of cells in the ventricular zone with radial processes that terminate with endfeet at the pial surface. These processes become fasciculated and attain maximal densities by E12-14 in the spinal cord and lower brainstem and by E14-16 in the midbrain, cerebellum and forebrain. Fasciculation is especially prominent for a subclass of these cells at the midline of the brainstem and spinal cord. As nuclear and cortical structures develop, the trajectories of the radial fiber fascicles undergo systematic and region-specific distortions in their initially simple linear configuration, in the process maintaining a consistent spatial registration of germinal ventricular zones with distal sites of assembly of post-migratory neurons. In the late fetal period, radial glial progressively disappear and scattered immature astrocytes bearing multiple fine processes appear in most regions of the CNS. In the spinal cord, a transitional unipolar radial form is identified in the emerging ventral and lateral funiculi between E13 and E17. In the cerebellum, precursors to the unipolar Bergmann glial cell are identified by E15, and in the retina, precursors of the bipolar Müller cell are identified by E16. Postnatally, RC1-stained radial glia become sparse, and after one week, immunoreactive cells include only ependymal cells, hypothalamic tanycytes, Bergmann glia, Müller cells, a unipolar radial form in the dentate gyrus, and a subpopulation of white matter astrocytes. These results suggest that radial cells of astroglial lineage comprise a diverse set of cell classes which subserve multiple functions in the developing and adult brain.
已产生一种单克隆抗体RC1，它可作为小鼠中枢神经系统（CNS）发育过程中放射状胶质细胞及相关细胞形态的选择性和灵敏免疫组织化学标记物。从胚胎第10天（E10）开始，对冰冻切片进行免疫细胞化学检测，结果显示整个中枢神经系统的脑室区有一群细胞被染色，这些细胞具有放射状突起，其终足止于软膜表面。这些突起在脊髓和低位脑干中于E12 - 14时开始成束并达到最大密度，在中脑、小脑和前脑则于E14 - 16时达到最大密度。在脑干和脊髓中线处的这类细胞的一个亚类中，成束现象尤为明显。随着核和皮质结构的发育，放射状纤维束的轨迹在其最初简单的线性构型中经历系统性和区域特异性的扭曲，在此过程中保持生发脑室区与迁移后神经元聚集的远端部位之间一致的空间定位。在胎儿后期，放射状胶质细胞逐渐消失，在中枢神经系统的大多数区域出现带有多个细突起的散在未成熟星形胶质细胞。在脊髓中，在E13至E17期间，在新出现的腹侧和外侧索中可识别出一种过渡性单极放射状形态。在小脑中，单极伯格曼胶质细胞的前体在E15时可被识别，在视网膜中，双极米勒细胞的前体在E16时可被识别。出生后，RC1染色的放射状胶质细胞变得稀疏，一周后，免疫反应性细胞仅包括室管膜细胞、下丘脑伸长细胞、伯格曼胶质细胞、米勒细胞、齿状回中的一种单极放射状形态以及白质星形胶质细胞的一个亚群（译者注：此处white matter astrocytes直译为白质星形胶质细胞，文中未明确其亚群具体特征，推测可能是具有某种特殊标记或功能的白质星形胶质细胞亚群）。这些结果表明，星形胶质细胞谱系的放射状细胞包括多种细胞类型，它们在发育中的大脑和成年大脑中发挥多种功能。
