Misson J P, Edwards M A, Yamamoto M, Caviness V S
Eunice Kennedy Shriver Center, Department of Developmental Neurobiology, Waltham, MA 02254.
Brain Res Dev Brain Res. 1988 Nov 1;44(1):95-108. doi: 10.1016/0165-3806(88)90121-6.
The monoclonal antibody RC2 was generated in mouse by conventional hybridoma methodology. The antigen recognized by RC2 is robust, allowing aldehyde fixation appropriate to high resolution light and electron microscopic analyses. From the neural tube stage of fetal development the antibody delineates throughout the central nervous system a subpopulation of neuroepithelial cells which have a radial bipolar morphology. A descending process extends to the ventricular margin, and an ascending process contacts the glial limiting membrane by one or more endfeet varicosities. The persistence of these cells through the neurogenetic period allows their identification as radial glial. From as early as E9-10 the fibers appear to be organized in simple straight fascicles. Later in fetal development these fascicles show marked region-specific transformations in density and trajectory, particularly in association with cerebral corticogenesis and with cerebellar and basal ganglia development. The bipolar forms continue to stain with RC2 until they disappear in the postnatal period. Concurrently with a progressive perinatal loss of stained bipolar radial glia, RC2 identifies multipolar cell forms at various levels of the brain wall, as consistent with the transformation of radial glia into astrocytes. RC2 also recognizes monopolar cell forms in the spinal cord and the cerebellum as early as E15, and in the dentate gyrus of the hippocampal formation from the day of birth. Monopolar forms in the cerebellum are inferred to be progenitors of Bergmann glia. Although Bergmann glia are known to persist in adult life, these cells do not stain with RC2 beyond the 2nd postnatal week. The robustness of the antigen recognized by RC2 makes this probe a valuable tool to study the morphological transformations of the bipolar radial glia during their mitotic turnover. It also provides a sensitive stain for the study of the organization and the histogenetic role of the overall radial fiber system.
单克隆抗体RC2是通过传统的杂交瘤技术在小鼠体内产生的。RC2识别的抗原稳定，适用于高分辨率光镜和电镜分析。从胎儿发育的神经管阶段开始，该抗体在整个中枢神经系统中勾勒出一群具有放射状双极形态的神经上皮细胞。一个下行突起延伸至脑室边缘，一个上行突起通过一个或多个终足曲张与胶质界膜接触。这些细胞在神经发生期持续存在，使其被鉴定为放射状胶质细胞。早在E9-10时，纤维似乎就以简单的直束状排列。在胎儿发育后期，这些束在密度和轨迹上表现出明显的区域特异性变化，特别是与大脑皮质发生以及小脑和基底神经节发育相关。双极形态的细胞在出生后消失前一直能用RC2染色。随着围产期染色的双极放射状胶质细胞逐渐减少，RC2识别出脑壁不同水平的多极细胞形态，这与放射状胶质细胞向星形胶质细胞的转化一致。RC2早在E15时就在脊髓和小脑中识别出单极细胞形态，从出生日起就在海马结构的齿状回中识别出单极细胞形态。小脑中的单极形态被推断为伯格曼胶质细胞的祖细胞。虽然已知伯格曼胶质细胞在成年期持续存在，但这些细胞在出生后第二周后就不再能用RC2染色。RC2识别的抗原的稳定性使该探针成为研究双极放射状胶质细胞在有丝分裂更新过程中形态转变的有价值工具。它还为研究整个放射状纤维系统的组织和组织发生作用提供了一种灵敏的染色方法。
