Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest, Romania.
Analyst. 2012 Feb 21;137(4):903-9. doi: 10.1039/c2an15892b. Epub 2011 Dec 13.
Monocrystalline diamond (natural diamond, synthetic-1 and synthetic-2) based electrochemical electrodes were designed for the analysis of L- and D-fucose. Response characteristics of the electrochemical electrodes were determined using cyclic voltammetry and differential pulse voltammetry (DPV). L-fucose was determined using DPV with electrodes based on natural diamond, synthetic-1 and synthetic-2, respectively, at 240 mV using NaCl as the electrolyte (pH 3.0); at 160 mV using KNO(3) (pH 10.0) and at 80 mV using KCl as the electrolyte (pH 10.0) while D-fucose was analyzed at 120 mV using KCl as the electrolyte (pH 1.0); at 140 mV using KNO(3) as the electrolyte (pH 1.0) and at 160 mV using NaNO(3) as the electrolyte (pH 3.0). The linear concentration ranges for L-fucose were between 10(-13) and 10(-9) mol L(-1) (natural diamond), 10(-11) and 10(-8) mol L(-1) (synthetic-1) and 10(-6) and 10(-3) mol L(-1) (synthetic-2) with detection limits of 10(-14), 10(-12) and 10(-8) mol L(-1) magnitude order, respectively. For D-fucose, the linear concentration ranges were 10(-6) to 10(-3) mol L(-1) (natural diamond), 10(-5) to 10(-3) mol L(-1) (synthetic-1) and 10(-9) to 10(-3) mol L(-1) (synthetic-2) with detection limits of 10(-7), 10(-7) and 10(-10) mol L(-1) magnitude order, respectively. The sensors were used for the assay of L-fucose in serum and urine samples.
基于单晶金刚石（天然金刚石、合成-1 和合成-2）的电化学电极被设计用于 L-和 D-岩藻糖的分析。使用循环伏安法和差分脉冲伏安法（DPV）来确定电化学电极的响应特性。使用基于天然金刚石、合成-1 和合成-2 的电极，分别在 240 mV 下使用 NaCl 作为电解质（pH 3.0）；在 160 mV 下使用 KNO₃（pH 10.0）和在 80 mV 下使用 KCl 作为电解质（pH 10.0）测定 L-岩藻糖，而 D-岩藻糖则在 120 mV 下使用 KCl 作为电解质（pH 1.0）；在 140 mV 下使用 KNO₃作为电解质（pH 1.0）和在 160 mV 下使用 NaNO₃作为电解质（pH 3.0）进行分析。L-岩藻糖的线性浓度范围分别为 10(-13) 到 10(-9) mol L(-1)（天然金刚石）、10(-11) 到 10(-8) mol L(-1)（合成-1）和 10(-6) 到 10(-3) mol L(-1)（合成-2），检测限分别为 10(-14)、10(-12)和 10(-8) mol L(-1)数量级。对于 D-岩藻糖，线性浓度范围分别为 10(-6) 到 10(-3) mol L(-1)（天然金刚石）、10(-5) 到 10(-3) mol L(-1)（合成-1）和 10(-9) 到 10(-3) mol L(-1)（合成-2），检测限分别为 10(-7)、10(-7)和 10(-10) mol L(-1)数量级。该传感器用于血清和尿液样品中 L-岩藻糖的测定。
