Stefan-van Staden Raluca-Ioana, Gugoaşă Livia Alexandra, Calenic Bogdan, Legler Juliette
1] Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania [2] Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
Sci Rep. 2014 Jul 4;4:5579. doi: 10.1038/srep05579.
Stochastic microsensors based on diamond paste and three types of electroactive materials (maltodextrin (MD), α-cyclodextrin (α-CD) and 5,10,15,20-tetraphenyl-21H,23H porphyrin (P)) were developed for the assay of estradiol (E2), testosterone (T2) and dihydrotestosterone (DHT) in children's saliva. The main advantage of utilization of such tools is the possibility to identify and quantify all three hormones within minutes in small volumes of childen's saliva. The limits of quantification obtained for DHT, T2, and E2 (1 fmol/L for DHT, 1 pmol/L for T2, and 66 fmol/L for E2) determined using the proposed tools allows the utilization of these new methods with high reliability for the screening of saliva samples from children. This new method proposed for the assay of the three hormones overcomes the limitations (regarding limits of determination) of ELISA method which is the standard method used in clinical laboratories for the assay of DHT, T2, and E2 in saliva samples. The main feature of its utilization for children's saliva is to identify earlier problems related to early puberty and obesity.
基于金刚石糊和三种电活性材料（麦芽糊精（MD）、α-环糊精（α-CD）和5,10,15,20-四苯基-21H,23H卟啉（P））开发了随机微传感器，用于检测儿童唾液中的雌二醇（E2）、睾酮（T2）和双氢睾酮（DHT）。使用此类工具的主要优点是能够在几分钟内对少量儿童唾液中的所有三种激素进行鉴定和定量。使用所提出的工具测定得到的DHT、T2和E2的定量限（DHT为1 fmol/L，T2为1 pmol/L，E2为66 fmol/L）使得这些新方法能够高度可靠地用于筛查儿童唾液样本。所提出的用于检测这三种激素的新方法克服了ELISA方法（临床实验室用于检测唾液样本中DHT、T2和E2的标准方法）在测定限方面的局限性。其用于儿童唾液检测的主要特点是能够更早地发现与青春期早熟和肥胖相关的问题。
