Galant S P, Underwood S, Allred S, Hanifin J M
J Invest Dermatol. 1979 Jun;72(6):330-2. doi: 10.1111/1523-1747.ep12531768.
It has been postulated that the patient with atopic dermatitis has defective beta adrenergic receptor function. However, a more generalized defect is suggested by the observation that cyclic AMP generation is diminished in these patients following stimulation with both isoproterenol and PGE1. To determine the nature of this abnormality, we measured beta adrenergic receptor binding directly on polymorphonuclear leukocyte membranes using the radiolabeled beta adrenergic antagonist (-) [3H]dihydroalprenolol (DHA). DHA binding was studied in 6 mild and 9 moderate-to-severe atopic dermatitis patients, and 8 normal controls using a subsaturating concentration of DHA (0.5 nM) to estimate receptor affinity and a saturating concentration of DHA (30 mM) to determine the total number of receptors per cell. No significant differences (p greater than .05) were found in the total number of receptors per PMN between the control population (805 +/- 95) and the mild atopic dermatitis patients (745 +/- 91) or the moderate to severe group (621 +/- 79). In addition, no significant differences in receptor affinity were found among any of the 3 study groups. These findings suggest that beta receptor binding in atopic dermatitis is normal. Reduced cyclic AMP generation in atopic dermatitis PMN leukocytes would appear to be due to a defect distal to the beta adrenergic receptor itself.
据推测，特应性皮炎患者存在β肾上腺素能受体功能缺陷。然而，异丙肾上腺素和前列腺素E1刺激后这些患者的环磷酸腺苷生成减少，这一观察结果提示存在更普遍的缺陷。为了确定这种异常的性质，我们使用放射性标记的β肾上腺素能拮抗剂（-）[3H]二氢心得舒（DHA）直接测量多形核白细胞膜上的β肾上腺素能受体结合情况。在6名轻度和9名中度至重度特应性皮炎患者以及8名正常对照中研究了DHA结合情况，使用亚饱和浓度的DHA（0.5 nM）来估计受体亲和力，使用饱和浓度的DHA（30 mM）来确定每个细胞的受体总数。对照人群（805±95）与轻度特应性皮炎患者（745±91）或中度至重度组（621±79）之间，每个多形核白细胞的受体总数没有显著差异（p＞0.05）。此外，三个研究组中的任何一组之间在受体亲和力方面均未发现显著差异。这些发现表明特应性皮炎中的β受体结合是正常的。特应性皮炎多形核白细胞中环磷酸腺苷生成减少似乎是由于β肾上腺素能受体本身远端的缺陷所致。
