Research Centre for Biocompatibility and Tissue Engineering, Department of Experimental and Clinical Medicine, University of Eastern Piedmont A. Avogadro, Novara, Italy.
J Mater Sci Mater Med. 2012 Feb;23(2):565-71. doi: 10.1007/s10856-011-4519-9. Epub 2011 Dec 13.
Ionic hydrogels are biocompatible candidates for skin tissue engineering. Two hydrogels synthesized by crosslinking gelatin with polylysine (positively charged HG1) or polyglutamic acid (negatively charged HG2) were tested using spontaneously immortalized human keratinocytes (HaCaT). HaCaT cells displayed higher adhesion and proliferation onto HG2, forming a continuous and stratified epithelium after 7 days. Moreover HaCaT cells grown onto HG2 showed a decreased Epilysin and Filaggrin expression, while transglutaminase-1 expression was increased. Those data indicate that human keratinocyte can form a stratified epithelium onto HG2 that could therefore be an useful tool for skin tissue engineering.
离子水凝胶是用于皮肤组织工程的生物相容性候选材料。通过将明胶与聚赖氨酸（带正电荷的 HG1）或聚谷氨酸（带负电荷的 HG2）交联合成了两种水凝胶，并使用自发永生化的人角质形成细胞（HaCaT）进行了测试。HaCaT 细胞在 HG2 上表现出更高的黏附和增殖能力，在 7 天后形成连续的分层上皮。此外，在 HG2 上生长的 HaCaT 细胞显示出 Epilysin 和 Filaggrin 表达降低，而转谷氨酰胺酶-1 表达增加。这些数据表明，人角质形成细胞可以在 HG2 上形成分层上皮，因此它可能是皮肤组织工程的有用工具。
