a Department of Tissue Engineering and Applied Cell Sciences , School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran , Iran.
b Division of Chemical Engineering, Department of Materials Engineering Science , Graduate School of Engineering Science, Osaka University , Toyonaka , Osaka , Japan.
J Biomater Sci Polym Ed. 2019 Mar;30(4):295-307. doi: 10.1080/09205063.2018.1562637. Epub 2019 Jan 31.
Hydrogel microcapsules having the ability to promote cell adhesion and proliferation are a useful tool for fabricating tissue in vitro. The present study explored the effects of two anionic polysaccharide hydrogel membranes which have an impact on the adhesiveness, morphology and growth of cells. Microcapsules were made by coating a cell-laden gelatin microparticle with a hydrogel membrane produced from modified hyaluronic acid or alginate possessing phenolic hydroxyl moieties (HA-Ph or Alg-Ph respectively) via a horseradish peroxidase-catalyzed crosslinking reaction. Some gelatin was retained within the microcapsules to support the attachment and growth of encapsulated cells. The morphological and functional characteristics of encapsulated HeLa and 10T1/2 cells were evaluated. The HA-Ph hydrogel, which exhibited greater retention of gelatin, showed a higher degree of cytocompatibility with respect to cell adhesion, spreading and proliferation compared with the Alg-Ph hydrogel membrane. These findings indicate that HA-Ph microcapsules synthesized around a temporary gelatin microparticles are a promising cell vehicle for tissue engineering applications.
具有促进细胞黏附和增殖能力的水凝胶微胶囊是体外构建组织的有用工具。本研究探讨了两种带负电荷的多糖水凝胶膜对细胞黏附性、形态和生长的影响。通过辣根过氧化物酶催化的交联反应，用含有酚羟基的改性透明质酸或海藻酸钠（分别为 HA-Ph 或 Alg-Ph）水凝胶膜包裹载细胞的明胶微球，制备微胶囊。一些明胶保留在微胶囊内，以支持包封细胞的附着和生长。评估了包封的 HeLa 和 10T1/2 细胞的形态和功能特征。与 Alg-Ph 水凝胶膜相比，具有更高明胶保留率的 HA-Ph 水凝胶在细胞黏附、铺展和增殖方面表现出更高的细胞相容性。这些发现表明，围绕临时明胶微球合成的 HA-Ph 微胶囊是组织工程应用中很有前途的细胞载体。
