Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Virol Sin. 2011 Dec;26(6):409-17. doi: 10.1007/s12250-011-3216-7. Epub 2011 Dec 10.
A bacterial cell surface display technique based on an ice nucleation protein has been employed for the development of live vaccine against viral infection. Due to its ubiquitous ability to invade host cells, Salmonella typhimurium might be a good candidate for displaying viral antigens. We demonstrated the surface display of domain III of Japanese encephalitis virus E protein and the enhanced green fluorescent protein on S. typhimurium BRD509 using the ice nucleation protein. The effects of the motif in the ice nucleation protein on the effective display of integral protein were also investigated. The results showed that display motifs in the protein can target integral foreign protein on the surface of S. typhimurium BRD509. Moreover, recombinant strains with surface displayed viral proteins retained their invasiveness, suggesting that the recombinant S. typhimurium can be used as live vaccine vector for eliciting complete immunogenicity. The data may yield better understanding of the mechanism by which ice nucleation protein displays foreign proteins in the Salmonella strain.
基于冰核蛋白的细菌表面展示技术已被用于开发针对病毒感染的活疫苗。由于其普遍具有侵袭宿主细胞的能力，鼠伤寒沙门氏菌可能是展示病毒抗原的良好候选者。我们利用冰核蛋白展示了日本脑炎病毒 E 蛋白结构域 III 和增强型绿色荧光蛋白在鼠伤寒沙门氏菌 BRD509 上的表面展示。还研究了冰核蛋白中的模体对整合蛋白有效展示的影响。结果表明，蛋白中的展示模体可将完整的外来蛋白靶向鼠伤寒沙门氏菌 BRD509 的表面。此外，具有表面展示病毒蛋白的重组菌株保留了其侵袭性，表明重组鼠伤寒沙门氏菌可用作活疫苗载体以引发完全免疫原性。这些数据可能有助于更好地理解冰核蛋白在沙门氏菌中展示外来蛋白的机制。
