Larsson P G, Platz-Christensen J J
Department of Obstetrics and Gynecology, Central Hospital, Skövde, Sweden.
Obstet Gynecol. 1990 Oct;76(4):727-30.
Among 235 women attending an outpatient clinic, the diagnosis of bacterial vaginosis was made using three of the following four criteria: typical discharge, pH more than 4.5, positive amine sniff test, and clue cells in a wet smear. These findings were correlated with the finding of clue cells in air-dried wet smears rehydrated more than 1 month after the visit. The rehydrated specimens had the same microscopic appearance as a nonpreserved wet smear. The demonstration of clue cells in the rehydrated smears correlated with the composite diagnosis of bacterial vaginosis with a sensitivity of 96% and specificity of 98%. In a busy multi-physician setting, the scoring of clue cells in stored and rehydrated smears can be used to obtain consistent readings for wet-smear diagnostics, thus simplifying teaching and increasing the utility of wet smears in clinical research.
在235名前往门诊就诊的女性中，采用以下四项标准中的三项来诊断细菌性阴道病：典型分泌物、pH值大于4.5、胺试验阳性以及湿涂片中有线索细胞。这些结果与就诊1个月多后复水的风干湿涂片中线索细胞的发现相关。复水后的标本具有与未保存的湿涂片相同的显微镜下外观。复水涂片中线索细胞的显示与细菌性阴道病的综合诊断相关，敏感性为96%，特异性为98%。在忙碌的多医生环境中，对储存和复水涂片中线索细胞进行评分可用于获得湿涂片诊断的一致读数，从而简化教学并提高湿涂片在临床研究中的实用性。
