Department of Materials Engineering and Industrial Technologies and Biotech Research Centre, University of Trento, Italy.
J Tissue Eng Regen Med. 2013 Feb;7(2):161-8. doi: 10.1002/term.516. Epub 2011 Dec 12.
Bone tissue regeneration involves different healing stages and the resulting final hard tissue is formed from natural templates such as fibrous collagen, soft and hard callus and capillary bed. This work aims to evaluate the efficiency of different scaffold geometries with a novel approach: exploring the relationships among scaffold morphologies, cell activity and collagen 3D organization, which serves as a natural template for subsequent mineralization. Among the possible systems to fabricate scaffolds, solvent casting with particulate leaching and microfabrication were used to produce random vs ordered structures from poly(D,L-lactic acid). In vitro biological testing was carried out by culturing a human osteosarcoma-derived osteoblast cell line (MG63) and measuring material cytotoxicity, cell proliferation and migration. Assemblage of collagen fibres was evaluated. A preliminary study of collagen distribution over the two different matrices was performed by confocal laser microscopy after direct red 80 staining. Both of the scaffolds were seen to be a good substrate for cell attachment, growth and proliferation. However, it seems that random, rather than regular, well-ordered porosity induces a more proper collagen fibre distribution and organization, similar to the natural one formed in the early stages of bone repair.
骨组织再生涉及不同的愈合阶段，最终的硬组织是由天然模板形成的，如纤维状胶原蛋白、软硬痂和毛细血管床。本工作旨在通过一种新的方法来评估不同支架几何形状的效率：探索支架形态、细胞活性和 3D 胶原组织之间的关系，后者作为随后矿化的天然模板。在可能的制造支架的系统中，使用溶剂浇铸与颗粒沥滤和微制造来从聚(D,L-乳酸)生产随机和有序结构。通过培养人骨肉瘤衍生成骨细胞系（MG63）并测量材料细胞毒性、细胞增殖和迁移，进行体外生物学测试。评估胶原纤维的聚集。通过直接红 80 染色后的共聚焦激光显微镜对两种不同基质上的胶原分布进行了初步研究。两种支架都被认为是细胞附着、生长和增殖的良好基质。然而，似乎随机的、而非规则的、有序的孔隙率会诱导更合适的胶原纤维分布和组织，类似于在骨修复的早期阶段形成的天然组织。
