Department of Industrial Engineering and Biotech Research Center, University of Trento, Italy.
Biomaterials. 2013 Jun;34(19):4573-81. doi: 10.1016/j.biomaterials.2013.02.009. Epub 2013 Mar 19.
Attainment of functional vascularization of engineered constructs is one of the fundamental challenges of tissue engineering. However, the development of an extracellular matrix in most tissues, including bone, is dependent upon the establishment of a well developed vascular supply. In this study a poly(d,l-lactic acid) (PDLLA) salt-leached sponge was modified by incorporation of silk fibroin fibers to create a multicomponent scaffold, in an effort to better support endothelial cell colonization and to promote in vivo vascularization. Scaffolds with and without silk fibroin fibers were compared for microstructure, mechanical properties, ability to maintain cell populations in vitro as well as to permit vascular ingrowth into acellular constructs in vivo. We demonstrated that adding silk fibroin fibers to a PDLLA salt-leached sponge enhanced scaffold properties and heightened its capacity to support endothelial cells in vitro and to promote vascularization in vivo. Therefore refinement of scaffold properties by inclusion of materials with beneficial attributes may promote and shape cellular responses.
实现工程化构建体的功能性血管化是组织工程的基本挑战之一。然而，包括骨骼在内的大多数组织中细胞外基质的形成都依赖于完善的血管供应。在这项研究中，我们通过加入丝素纤维对聚（DL-乳酸）（PDLLA）盐析海绵进行了改性，以创建一个多组分支架，以更好地支持内皮细胞的定植并促进体内血管生成。比较了具有和不具有丝素纤维的支架的微观结构、机械性能、在体外维持细胞群体的能力以及允许血管长入无细胞构建体的能力。我们证明，向 PDLLA 盐析海绵中添加丝素纤维可增强支架的性能，并提高其在体外支持内皮细胞的能力和促进体内血管生成的能力。因此，通过包含具有有益特性的材料来改善支架性能可能会促进和塑造细胞反应。
