Aslan Kadir
Morgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251.
Nano Biomed Eng. 2011;3(3):179-183. doi: 10.5101/nbe.v3i3.p179-183.
We present the rapid and sensitive detection of Troponin I-T-C (Tn I-T-C) complex from buffer and human serum samples using Microwave-Accelerated and Metal-Enhanced Fluorescence (MA-MEF) technique, which is based on the combined use of low power microwave heating, silver nanoparticle films (SNFs) and fluorescence spectroscopy. The detection of Tn I-T-C complex from buffer solutions and human serum samples on SNFs was carried out using fluorescence-based immunoassays at room temperature (control immunoassay, 2 hour total assay time) and using low-power microwave heating (MA-MEF-based immunoassay, 1 minute total assay time). A lower detection limit for Tn I-T-C complex from buffer solutions in the control immunoassay and MA-MEF-based immunoassay was 0.01 ng/ml and 0.005 ng/ml, respectively. However, the lower detection limit for Tn I-T-C complex from human serum in the control immunoassay was increased to 10 ng/ml. The use of MA-MEF technique afforded for the detection of Tn I-T-C complex from human serum samples in 1 min with a lower detection limit of 0.05 ng/ml.
我们展示了使用微波加速和金属增强荧光（MA-MEF）技术从缓冲液和人血清样本中快速灵敏地检测肌钙蛋白I-T-C（Tn I-T-C）复合物，该技术基于低功率微波加热、银纳米颗粒薄膜（SNFs）和荧光光谱的联合使用。在室温下（对照免疫测定，总测定时间2小时），使用基于荧光的免疫测定法，并使用低功率微波加热（基于MA-MEF的免疫测定法，总测定时间1分钟），在SNFs上对缓冲液溶液和人血清样本中的Tn I-T-C复合物进行检测。对照免疫测定法和基于MA-MEF的免疫测定法中，缓冲液溶液中Tn I-T-C复合物的检测下限分别为0.01 ng/ml和0.005 ng/ml。然而，对照免疫测定法中，人血清中Tn I-T-C复合物的检测下限提高到了10 ng/ml。使用MA-MEF技术能够在1分钟内检测人血清样本中的Tn I-T-C复合物，检测下限为0.05 ng/ml。
