Okuda-Shimazaki Junko, Takaku Saiko, Kanehira Koki, Sonezaki Shunji, Taniguchi Akiyohshi
Advanced Medical biomaterials Group, Biomaterials Center, National Institute for Materials Science (NIMS)/1-1, Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Int J Mol Sci. 2010 Jun 7;11(6):2383-92. doi: 10.3390/ijms11062383.
Titanium dioxide (titania) nanoparticle aggregation is an important factor in understanding cytotoxicity. However, the effect of the aggregate size of nanoparticles on cells is unclear. We prepared two sizes of titania aggregate particles and investigated their biological activity by analyzing biomarker expression based on mRNA expression analysis. The aggregate particle sizes of small and large aggregated titania were 166 nm (PDI = 0.291) and 596 nm (PDI = 0.417), respectively. These two size groups were separated by centrifugation from the same initial nanoparticle sample. We analyzed the gene expression of biomarkers focused on stress, inflammation, and cytotoxicity. Large titania aggregates show a larger effect on cell viability and gene expression when compared with the small aggregates. This suggests that particle aggregate size is related to cellular effects.
二氧化钛纳米颗粒的聚集是理解细胞毒性的一个重要因素。然而，纳米颗粒聚集体大小对细胞的影响尚不清楚。我们制备了两种大小的二氧化钛聚集体颗粒，并通过基于mRNA表达分析的生物标志物表达分析来研究它们的生物活性。小尺寸和大尺寸二氧化钛聚集体颗粒的粒径分别为166 nm（多分散指数PDI = 0.291）和596 nm（PDI = 0.417）。这两个尺寸组是通过离心从相同的初始纳米颗粒样品中分离出来的。我们分析了关注应激、炎症和细胞毒性的生物标志物的基因表达。与小聚集体相比，大尺寸二氧化钛聚集体对细胞活力和基因表达的影响更大。这表明颗粒聚集体大小与细胞效应有关。
