[江苏省中华按蚊对杀虫剂的敏感性]

[Sensitivity of Anopheles sinensis to insecticides in Jiangsu Province].

作者信息

Li Ju-Lin, Zhou Hua-Yun, Cao Jun, Zhu Guo-Ding, Wang Wei-Ming, Gu Yaping, Liu Yaobao, Cao Yuanyuan, Zhang Chao, Gao Qi

机构信息

Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Jun;23(3):296-300.

PMID:22164497

Abstract

OBJECTIVE

To understand the sensitivity of Anopheles sinensis to deltamethrin, DDT and malathion in Jiangsu province.

METHODS

The adult mosquitoes were captured from the fields of Sihong, Yangzhong and Yixing counties (cities) and cultured, and by using the method recommended by WHO, their first filial generations knocked down at 10, 15, 20, 30, 40, 50, and 60 min after exposure with insecticides aforementioned were counted, and the mortality of mosquitoes tested after 24 h was recorded.

RESULTS

The knock down rates of mosquitoes, which were the first generation (F0) of Anopheles sinensis captured from the field of Sihong, Yangzhong, Yixing counties (cities) to 0.05% deltamethrin were 28.57%, 57.14%, 52.38%, respectively 60 min after the exposure; and the mortality rates 24 h-post-exposure were 35.71%, 57.14%, 61.90%, respectively. The resistance degree to deltamethrin was assessed as "R" level. The knock down rates of mosquitoes 60 min after the exposure to 4% DDT were 9.52%, 2.38%, 4.76%, respectively, and the mortality rates 24 h-post-exposure were 47.62%, 50.00%, 40.48%, respectively. The resistance degree to DDT was assessed as "R" level. The knock down rates of mosquitoes 60 min after the exposure to 5% malathion were 11.90%, 28.57%, 28.13%, respectively, and the mortality rates 24 h after the exposure were 80.95%, 85.71%, 93.75%, respectively. The resistance degree to malathion was assessed as level "M". The knock down rates of Anopheles sinensis captured in day 1, day 7, day 15 and the F1 from Yixing 60 min after the exposure to 0.05% deltamethrin were 54.76%, 76.19%, 92.86%, and 52.38%, respectively, and the mortality rates post-24 h were 54.76%, 76.19%, 95.23% and 61.90%, respectively. The difference of mortality post-24 h between the mosquitoes of 1 day post-captured and F1 was not statistically significant (P > 0.05). The knock down rates 60 min after the exposure to 0.05% deltamethrin to the female and male F1 of Anopheles sinensis from Sihong were 28.57% and 40.48%, and the mortality rates post-24 h were 35.71%, 42.86% respectively, and the difference was not statistically significant (P > 0.05).

CONCLUSIONS

Anopheles sinensis in the field of Jiangsu Province has developed severe resistance to deltamethrin and DDT, initial resistance to malathion. In order to prevent the development of resistance to the insecticides, the integrated management measures should be adopted in the future.

摘要

目的

了解江苏省中华按蚊对溴氰菊酯、滴滴涕和马拉硫磷的敏感性。

方法

从泗洪、扬中、宜兴县（市）的田间捕获成蚊并进行饲养，采用世界卫生组织推荐的方法，统计上述杀虫剂处理后10、15、20、30、40、50和60分钟时其第一代子代的击倒只数，并记录处理24小时后试虫的死亡率。

结果

泗洪、扬中、宜兴县（市）野外捕获的中华按蚊第一代（F0）对0.05%溴氰菊酯处理60分钟后的击倒率分别为28.57%、57.14%、52.38%；处理24小时后的死亡率分别为35.71%、57.14%、61.90%。对溴氰菊酯的抗性程度评定为“R”级。对4%滴滴涕处理60分钟后的击倒率分别为9.52%、2.38%、4.76%；处理24小时后的死亡率分别为47.62%、50.00%、40.48%。对滴滴涕的抗性程度评定为“R”级。对5%马拉硫磷处理60分钟后的击倒率分别为11.90%、28.57%、28.13%；处理24小时后的死亡率分别为80.95%、85.71%、93.75%。对马拉硫磷的抗性程度评定为“M”级。宜兴捕获的中华按蚊第1天、第7天、第15天及F1代对0.05%溴氰菊酯处理60分钟后的击倒率分别为54.76%、76.19%、92.86%和52.38%；处理24小时后的死亡率分别为54.76%、76.19%、95.23%和61.90%。捕获后1天的试虫与F1代处理24小时后的死亡率差异无统计学意义（P>0.05）。泗洪中华按蚊F1代雌、雄虫对0.05%溴氰菊酯处理60分钟后的击倒率分别为28.57%和40.48%；处理24小时后的死亡率分别为35.71%、42.86%，差异无统计学意义（P>0.05）。