Kiprianova E A, Klochko V V, Zelena L B, Churkina L N, Avdeeva L V
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Acad.Zabolotny St., Kyiv. MSP, 03680, Ukraine.
Mikrobiol Z. 2011 Sep-Oct;73(5):3-8.
Four novel strains of saprophytic bacteria were isolated from the soil samples collected in the moist subtropics region (the Black Sea coast of the Caucasus) and studied using methods of polyphasic taxonomic analysis. Microorganisms were Gram-negative, oxidase positive, aerobic, rod-shaped motile bacteria that produced antibiotic named batumin with high and selective activity against staphylococci; its total formula was C30H48N2O7 Phylogenetic analysis of 16S rRNA gene sequences (1376 bp, accession number in Genbank--JF306642) indicated that the isolates belonged to the gamma-Proteobacteria formed a separate branch within the genus Pseudomonas and had 98% 16S rRNA gene sequence similarity with Pseudomonas gingeri. The latter essentially differed from the studied strains in its phenotypic characteristics. The predominant cellular fatty acids of isolates were similar and included Cl6:0, C16:1, C18:1, and up to 22.9% of deltaC17:0; their DNA G+C content was 64.0 mol%. An analysis of taxonomic data indicated that the studied isolates represented a novel species, for which the name Pseudomonas batumici sp. nov. is proposed with the type strain UCM B-321 (Ukrainian Collection of Microorganisms).
从潮湿亚热带地区（高加索黑海沿岸）采集的土壤样本中分离出四株新的腐生细菌，并采用多相分类分析方法对其进行了研究。这些微生物为革兰氏阴性、氧化酶阳性、需氧、杆状运动细菌，产生了名为巴图明的抗生素，对葡萄球菌具有高选择性活性；其总分子式为C30H48N2O7。对16S rRNA基因序列（1376 bp，Genbank登录号——JF306642）的系统发育分析表明，分离株属于γ-变形菌纲，在假单胞菌属内形成一个单独分支，与生姜假单胞菌的16S rRNA基因序列相似性为98%。后者在表型特征上与所研究的菌株有本质区别。分离株的主要细胞脂肪酸相似，包括C16:0、C16:1、C18:1，以及高达22.9%的δC17:0；其DNA G+C含量为64.0 mol%。分类学数据分析表明，所研究的分离株代表一个新物种，为此提出名为巴图米假单胞菌（Pseudomonas batumici sp. nov.）的新名称，模式菌株为UCM B-321（乌克兰微生物菌种保藏中心）。
