Zuo Hui, Morimoto Yuh, Muroi Kenzo, Baba Tadashi
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama-shi, Tokyo, Japan.
Department of Radiological Technology, Faculty of Health Science, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Access Microbiol. 2024 Aug 16;6(8). doi: 10.1099/acmi.0.000799.v3. eCollection 2024.
Soil samples from a remote Japanese island (Kozushima) were processed and investigated for organisms exhibiting antimicrobial activity against pathogenic strains. A strain demonstrating antimicrobial activity against () was identified, prompting further investigation. Whole-genome sequencing was employed to identify the species and conduct phylogenetic analysis, followed by molecular analysis. Chemotaxonomic and biochemical analyses were conducted to further characterize the strain. Genomic analysis identified the strain of interest as (), originally isolated from soil of the Black Sea coast of the Caucasus in 1980. Koz11 is the second strain to be isolated and identified outside its initial area of discovery. Similar to the type strain, Koz11 showed antimicrobial activity against various strains, including methicillin-resistant (MRSA) and vancomycin-resistant (VRSA). However, the previously reported 'batumin gene cluster', which synthesizes antimicrobial compounds, was absent from Koz11. This study provides new insights into . Since the type strain of is exclusively deposited in the Ukrainian Collection of Microorganisms, the Koz11 strain may serve as a surrogate to facilitate continued study of .
对来自日本偏远岛屿（小笠原岛）的土壤样本进行了处理，并对其中对致病菌株具有抗菌活性的微生物进行了研究。鉴定出了一株对（）具有抗菌活性的菌株，从而促使进行进一步研究。采用全基因组测序来鉴定该物种并进行系统发育分析，随后进行分子分析。进行了化学分类学和生化分析以进一步表征该菌株。基因组分析确定所关注的菌株为（），该菌株最初于1980年从高加索黑海沿岸的土壤中分离得到。Koz11是在其最初发现区域之外分离和鉴定出的第二株（）菌株。与模式菌株相似，Koz11对多种（）菌株具有抗菌活性，包括耐甲氧西林金黄色葡萄球菌（MRSA）和耐万古霉素金黄色葡萄球菌（VRSA）。然而，Koz11中不存在先前报道的合成抗菌化合物的“巴图明基因簇”。本研究为（）提供了新的见解。由于（）的模式菌株仅保藏于乌克兰微生物菌种保藏中心，Koz11菌株可作为替代菌株，以促进对（）的持续研究。
