Ondrej Vladan, Havránek Pavel, Kitner Miloslav, Nemcová Pavla
Laboratory of Plant Tissue Cultures, Department of Botany, Faculty of Science, Palack, University, Slechtitelů 11, Olomouc 783 71, Czech Republic.
Int J Med Mushrooms. 2011;13(4):369-75. doi: 10.1615/intjmedmushr.v13.i4.70.
The accessions of the morel (Morchellaceae, Ascomycota) germplasm collection were genetically analyzed, in order to determine both their inter- and intraspecific relationships. This was done as a starting point for cultivation experiments, as well as to provide a genetic description of invasive morel populations linked to mulched garden patches, as compared with outdoor morels. The phylogenetic data, which was based on the internal transcribed spacer (ITS) sequences and supported by amplified fragment length polymorphism (AFLP) analyses, divided the germplasm isolates and accessions from the sequence database into three groups of yellow morels, and three groups of black morels, involving a remarkable monotypic genus of half-free morels (Mitrophora semilibera), the groups Morchella conica and M. angusticeps. Both Morchella groups include morel samples that use mulch bark as a vector for their spread across gardens in various locations in the Czech Republic. The AFLP analysis supported the ITS-based phylogenetic data and determined the intraspecific genetic profile of these, as a rule, almost entirely unstudied isolates.
对羊肚菌（羊肚菌科，子囊菌门）种质资源库中的种质进行了遗传分析，以确定它们的种间和种内关系。这样做是为了作为栽培实验的起点，同时与室外羊肚菌相比，对与覆盖花园地块相关的入侵羊肚菌种群进行遗传描述。基于内部转录间隔区（ITS）序列并由扩增片段长度多态性（AFLP）分析支持的系统发育数据，将序列数据库中的种质分离株和种质分为三组黄羊肚菌和三组黑羊肚菌，其中包括一个显著的单型属半开羊肚菌（Mitrophora semilibera）、圆锥羊肚菌组和尖顶羊肚菌组。两个羊肚菌组都包括一些羊肚菌样本，这些样本利用覆盖树皮作为载体，在捷克共和国不同地点的花园中传播。AFLP分析支持了基于ITS的系统发育数据，并确定了这些通常几乎完全未被研究的分离株的种内遗传特征。
