Shen Li-Jie, Li Wei, He Yan-Hong
Institute of Pathogens and Vectors, Dali University, Dali 671000, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Aug;23(4):465-7, 469.
This paper reviews the domestic and foreign literatures regarding molecular diagnosis of toxoplasmosis. The nucleic acid molecule hybridization and Toxoplasma gondii gene sequences for polymerase chain reaction (PCR), common and conventional PCR, nested PCR, real-time quantitative PCR, immune PCR, in-situ PCR, loop-mediated isothermal amplification technology are introduced.
本文综述了国内外有关弓形虫病分子诊断的文献。介绍了核酸分子杂交以及用于聚合酶链反应（PCR）的弓形虫基因序列，包括普通和常规PCR、巢式PCR、实时定量PCR、免疫PCR、原位PCR、环介导等温扩增技术。
