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弓形虫病的分子诊断：血沉棕黄层在检测循环中的刚地弓形虫方面的价值。

Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii.

作者信息

Brenier-Pinchart Marie-Pierre, Capderou Elodie, Bertini Rose-Laurence, Bailly Sébastien, Fricker-Hidalgo Hélène, Varlet-Marie Emmanuelle, Murat Jean-Benjamin, Sterkers Yvon, Touafek Fériel, Bastien Patrick, Pelloux Hervé

机构信息

Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France; Pôle "Biologie Moléculaire" du Centre National de Référence de la Toxoplasmose, Montpellier, France.

Laboratoire de Parasitologie et Mycologie, CHU de Grenoble et Université Grenoble Alpes, Grenoble 1 BP 217, 38043 Grenoble, France.

出版信息

Diagn Microbiol Infect Dis. 2015 Aug;82(4):289-91. doi: 10.1016/j.diagmicrobio.2015.04.004. Epub 2015 Apr 24.

DOI:10.1016/j.diagmicrobio.2015.04.004
PMID:25957458
Abstract

Early detection of Toxoplasma tachyzoites circulating in blood using PCR is recommended for immunosuppressed patients at high risk for disseminated toxoplasmosis. Using a toxoplasmosis mouse model, we show that the sensitivity of detection is higher using buffy coat isolated from a large blood volume than using whole blood for this molecular monitoring.

摘要

对于有播散性弓形虫病高风险的免疫抑制患者，建议使用聚合酶链反应（PCR）早期检测血液中循环的弓形虫速殖子。利用弓形虫病小鼠模型，我们发现，对于这种分子监测，从大量血液中分离出的血沉棕黄层比全血具有更高的检测灵敏度。

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