细胞色素 c 在油水界面作为生物膜模型的电子传递机制。

Electron transfer mechanism of cytochrome c at the oil/water interface as a biomembrane model.

机构信息

Department of Chemistry, Graduate School of Science, Kobe University, Nada, Kobe, Japan.

出版信息

J Phys Chem B. 2012 Jan 12;116(1):585-92. doi: 10.1021/jp2092658. Epub 2011 Dec 28.

DOI:10.1021/jp2092658

Abstract

The electron transfer (ET) between cytochrome c (Cyt c) in water (W) and 1,1'-dimethylferrocene (DiMFc) in 1,2-dichloroethane (DCE) was studied. The cyclic voltammograms obtained for the interfacial ET under various conditions could be well reproduced by digital simulation based on the ion-transfer (IT) mechanism, in which the ET process occurs not at the DCE/W interface but in the W phase nearest the interface. In this mechanism, the current signal is due to the IT of DiMFc(+) as the reaction product. On the other hand, the measurement of the double-layer capacity showed that Cyt c is adsorbed at the DCE/W interface. However, the contribution from the adsorbed proteins to the overall ET is considered to be small because of the thicker reaction layer in the IT mechanism. These findings would offer a useful suggestion for the behaviors of Cyt c in vivo.

摘要

电子转移（ET）在水（W）中的细胞色素 c（Cyt c）和 1,1'-二甲基二茂铁（DiMFc）在 1,2-二氯乙烷（DCE）之间进行了研究。在各种条件下，界面 ET 的循环伏安图可以通过基于离子转移（IT）机制的数字模拟很好地再现，其中 ET 过程不是在 DCE/W 界面发生，而是在界面附近的 W 相中发生。在该机制中，电流信号是由于反应产物 DiMFc(+)的 IT 引起的。另一方面，双层电容的测量表明 Cyt c 被吸附在 DCE/W 界面上。然而，由于 IT 机制中的反应层较厚，吸附蛋白质对整体 ET 的贡献被认为较小。这些发现将为 Cyt c 在体内的行为提供有用的建议。