Osakai Toshiyuki
Department of Chemistry, Graduate School of Science, Kobe University.
Anal Sci. 2019 Apr 10;35(4):361-366. doi: 10.2116/analsci.18R005. Epub 2019 Jan 4.
Many studies have been conducted by using the oil (O) | water (W) interface as a simple model for understanding ion transfer (IT) or electron transfer (ET) across biomembranes. In this review, we revisit the usability of the O | W interface as a biomembrane model. For understanding biomembrane IT, the O | W interface is the simplest and best suited model. For example, the standard Gibbs transfer energy of drug ions at the O | W interface is a useful measure for evaluating their membrane permeability in a conventional in vitro assay, called PAMPA. However, the O | W interface is not necessarily a good model for understanding biomembrane ET. This is because no net current can be observed with the O | W interface, owing to the ET-coupled proton transfer. In such a case, the self-assembled monolayer (SAM) formed on a metal electrode serves as a better model for understanding biomembrane ET.
许多研究通过使用油（O）|水（W）界面作为简单模型来理解离子转移（IT）或电子转移（ET）穿过生物膜的过程。在本综述中，我们重新审视O|W界面作为生物膜模型的适用性。为了理解生物膜IT，O|W界面是最简单且最合适的模型。例如，药物离子在O|W界面的标准吉布斯转移能是在一种称为PAMPA的传统体外测定中评估其膜通透性的有用指标。然而，O|W界面不一定是理解生物膜ET的良好模型。这是因为由于ET耦合的质子转移，在O|W界面无法观察到净电流。在这种情况下，在金属电极上形成的自组装单分子层（SAM）是理解生物膜ET的更好模型。
