Department of Biochemistry, National University of Singapore, Singapore.
Biochem Biophys Res Commun. 2012 Jan 6;417(1):446-50. doi: 10.1016/j.bbrc.2011.11.136. Epub 2011 Dec 7.
Several phenolic compounds as well as ascorbate can oxidise in certain cell culture media (especially Dulbecco's modified Eagle's medium (DMEM)) to generate hydrogen peroxide. Addition of oxaloacetate decreased the levels of H(2)O(2) detected and the oxaloacetate was depleted. Oxaloacetate was approximately as effective as pyruvate in decreasing H(2)O(2) levels and more effective than α-ketoglutarate. Our data raise important issues to consider when interpreting the behaviour and metabolism of cells in culture (which are both altered by the oxidative stress of cell culture) and their apparent response to addition of autooxidisable compounds such as ascorbate and epigallocatechin gallate.
几种酚类化合物和抗坏血酸在某些细胞培养基（特别是杜尔贝科改良伊格尔培养基（DMEM））中会发生氧化，生成过氧化氢。添加草酰乙酸可以降低检测到的 H(2)O(2)水平，并且草酰乙酸被耗尽。草酰乙酸在降低 H(2)O(2)水平方面与丙酮酸大致相同，但比 α-酮戊二酸更有效。我们的数据在解释培养细胞的行为和代谢（细胞培养的氧化应激会改变它们）及其对自氧化化合物（如抗坏血酸和表没食子儿茶素没食子酸酯）的添加的明显反应时，提出了一些重要的问题需要考虑。
