Department of Biochemistry, National University of Singapore, Singapore.
Biochem Biophys Res Commun. 2011 Mar 4;406(1):20-4. doi: 10.1016/j.bbrc.2011.01.091. Epub 2011 Jan 31.
Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of α-ketoglutarate, which is known to be released by several cell types, decreased the levels of H(2)O(2), and the α-ketoglutarate was depleted and converted to succinate. These observations could account for previous reports of the protective effects of α-ketoglutarate in promoting the growth of cells in culture, and may contribute to explaining some of the variability in the literature in reported rates of H(2)O(2) production from autoxidisable compounds in cell culture systems.
抗坏血酸和几种酚类化合物在细胞培养液中很容易被氧化生成过氧化氢。然而，添加α-酮戊二酸（几种细胞类型都已知会释放出α-酮戊二酸）会降低 H(2)O(2)的水平，α-酮戊二酸被耗尽并转化为琥珀酸。这些观察结果可以解释先前关于α-酮戊二酸在促进细胞培养生长的保护作用的报道，并且可能有助于解释细胞培养系统中自氧化化合物产生 H(2)O(2)的报道率的文献中的一些可变性。
