白藜芦醇通过 SIRT1 依赖性机制防止皮质酮诱导的培养肌管中肌肉萎缩相关泛素连接酶 atrogin-1 和 MuRF1 的表达。
Resveratrol prevents dexamethasone-induced expression of the muscle atrophy-related ubiquitin ligases atrogin-1 and MuRF1 in cultured myotubes through a SIRT1-dependent mechanism.
机构信息
Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, United States.
出版信息
Biochem Biophys Res Commun. 2012 Jan 6;417(1):528-33. doi: 10.1016/j.bbrc.2011.11.154. Epub 2011 Dec 7.
Abstract
Resveratrol (3,5,4'-trihydroxystilbene) has been ascribed multiple beneficial biological effects but the influence of resveratrol on glucocorticoid-induced muscle atrophy is not known. We examined the effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression, FOXO1 acetylation, protein degradation and atrophy in cultured L6 myotubes. In addition, the role of the deacetylase SIRT1 in the effects of resveratrol was determined by transfecting myotubes with SIRT1 siRNA. The catabolic effects of dexamethasone were prevented by resveratrol and the protective effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression were abolished in myotubes transfected with SIRT1 siRNA. Results suggest that resveratrol can prevent glucocorticoid-induced muscle wasting and that this effect is at least in part SIRT1-dependent.
摘要
白藜芦醇（3,5,4'-三羟基二苯乙烯）具有多种有益的生物学作用，但白藜芦醇对糖皮质激素诱导的肌肉萎缩的影响尚不清楚。我们研究了白藜芦醇对地塞米松诱导的肌萎缩蛋白 1（atrogin-1）和肌肉环指蛋白 1（MuRF1）表达、FOXO1 乙酰化、蛋白降解和萎缩的影响。此外，通过肌管转染 SIRT1 siRNA 确定了去乙酰化酶 SIRT1 在白藜芦醇作用中的作用。白藜芦醇可预防地塞米松的分解代谢作用，而在转染 SIRT1 siRNA 的肌管中，白藜芦醇对地塞米松诱导的 atrogin-1 和 MuRF1 表达的保护作用被消除。结果表明，白藜芦醇可以预防糖皮质激素诱导的肌肉消耗，而这种作用至少部分依赖于 SIRT1。
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