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Ann Clin Lab Sci. 2011 Fall;41(4):353-9.
2
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本文引用的文献

1
Defining the role of the JAK-STAT pathway in head and neck and thoracic malignancies: implications for future therapeutic approaches.定义 JAK-STAT 通路在头颈部和胸部恶性肿瘤中的作用：对未来治疗方法的影响。
Drug Resist Updat. 2010 Jun;13(3):67-78. doi: 10.1016/j.drup.2010.04.001. Epub 2010 May 14.
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Is HPV-DNA testing a useful tool in predicting low-grade squamous intraepithelial lesion outcome? A retrospective longitudinal study.HPV-DNA 检测在预测低级别鳞状上皮内病变结局中的作用：一项回顾性纵向研究。
Int J Immunopathol Pharmacol. 2010 Jan-Mar;23(1):317-26. doi: 10.1177/039463201002300130.
3
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.白细胞介素（IL）-7及其受体（IL-7R）在霍奇金和里德-斯腾伯格细胞上的功能性共表达：IL-7参与霍奇金淋巴瘤的肿瘤细胞生长和微环境相互作用。
Int J Cancer. 2009 Sep 1;125(5):1092-101. doi: 10.1002/ijc.24389.
4
CD30-induced signaling is absent in Hodgkin's cells but present in anaplastic large cell lymphoma cells.CD30诱导的信号传导在霍奇金细胞中不存在，但在间变性大细胞淋巴瘤细胞中存在。
Am J Pathol. 2008 Feb;172(2):510-20. doi: 10.2353/ajpath.2008.070858. Epub 2008 Jan 10.
5
STAT5 is required for long-term maintenance of normal and leukemic human stem/progenitor cells.STAT5是正常和白血病人类干细胞/祖细胞长期维持所必需的。
Blood. 2007 Oct 15;110(8):2880-8. doi: 10.1182/blood-2006-08-039073. Epub 2007 Jul 13.
6
The expression of CD30 in anaplastic large cell lymphoma is regulated by nucleophosmin-anaplastic lymphoma kinase-mediated JunB level in a cell type-specific manner.间变性大细胞淋巴瘤中CD30的表达由核磷蛋白-间变性淋巴瘤激酶以细胞类型特异性方式介导的JunB水平调控。
Cancer Res. 2006 Sep 15;66(18):9002-8. doi: 10.1158/0008-5472.CAN-05-4101.
7
IL-7R alpha gene expression is inversely correlated with cell cycle progression in IL-7-stimulated T lymphocytes.白细胞介素-7受体α基因表达与白细胞介素-7刺激的T淋巴细胞的细胞周期进程呈负相关。
J Immunol. 2006 Jun 1;176(11):6702-8. doi: 10.4049/jimmunol.176.11.6702.
8
Cell biology of IL-7, a key lymphotrophin.关键淋巴细胞营养因子IL-7的细胞生物学
Cytokine Growth Factor Rev. 2005 Aug-Oct;16(4-5):513-33. doi: 10.1016/j.cytogfr.2005.05.004.
9
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Cancer. 2003 Oct 25;99(5):263-71. doi: 10.1002/cncr.11673.
10
Stability of archived liquid-based cervical cytologic specimens.存档液基宫颈细胞学标本的稳定性
Cancer. 2003 Apr 25;99(2):89-96. doi: 10.1002/cncr.11058.

在d1细胞、卡帕斯细胞和外周血单个核细胞长期储存后，通过液基细胞学检测核抗原和膜抗原。

Detection of nuclear and membrane antigens by liquid-based cytology following long-term storage of d1 cells, karpas cells, and peripheral blood mononuclear cells.

作者信息

Zappacosta Roberta, Aiello Francesca B, D'Antuono Tommaso, Procopio Antonio D, Durum Scott K, Conti Pio, Rosini Sandra

机构信息

Department of Oncology and Experimanetal Medicine, Section of Cytopathology "G. d'Annunzio" University of Chieti-Pescara Via dei Vestini, 66100 Chieti Scalo, Italy.

出版信息

Ann Clin Lab Sci. 2011 Fall;41(4):353-9.

PMID:22166505
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7370961/
Abstract

Immunofluorescence is the most frequently utilized technique to analyze protein expression. Fixed immunofluorescent cell suspensions, however, can only be stored for a week. We investigated whether liquid-based cytology could be used to detect antigens in cultured cells after a long storage period. Murine and human cells were fixed in PreservCyt solution, stored for various periods, and then used to perform an automated immunocytochemical analysis. Phosphorylation of the nuclear transcription factor Stat-5 induced by IL-7 was detected up to 4 months after IL-7 stimulation. Simultaneous nuclear positivity for the proliferation index MIB-1 and membrane positivity for the CD30 antigen were evident three months after fixation. Liquid-based cytology thus ensures long-lasting nuclear and membrane antigen immunoreactivity and permits the storage of cells from laborious experiments at room temperature for future analyses.

摘要

免疫荧光是分析蛋白质表达最常用的技术。然而，固定的免疫荧光细胞悬液只能保存一周。我们研究了液基细胞学是否可用于检测长期保存后的培养细胞中的抗原。将小鼠和人类细胞固定在PreservCyt溶液中，保存不同时间，然后用于进行自动免疫细胞化学分析。在IL-7刺激后长达4个月仍可检测到IL-7诱导的核转录因子Stat-5的磷酸化。固定三个月后，增殖指数MIB-1的核阳性和CD30抗原的膜阳性同时明显。因此，液基细胞学可确保核和膜抗原的长期免疫反应性，并允许将费力实验获得的细胞在室温下保存以备将来分析。