Ziora Katarzyna T, Oswiecimska Joanna M, Swietochowska Elzbieta, Ostrowska Zofia, Stojewska Małgorzata, Gorczyca Piotr, Rojewska Katarzyna, Ziora-Jakutowicz Karolina, Szczepanska Maria, Ziora Dariusz
Department of Paediatrics in Zabrze, Silesian University of Medicine in Katowice, Poland.
Neuro Endocrinol Lett. 2011;32(5):691-6.
There are only few studies available on blood resistin (RES) levels in patients with anorexia nervosa (AN), which revealed scarce results, however it has been demonstrated that RES mRNA expression in adipose tissue of these patients is increased. The aim of this study is: 1) the evaluation of serum resistin levels in girls with AN and determination a threshold value differentiating these patients from healthy subjects; 2) analysis of the relationship between serum resistin levels and BMI in examined subjects.
Serum RES concentration has been assayed using ELISA kit in 195 adolescent girls: 87 with restrictive AN (mean BMI-SDS: -2.65 ± 0.2), 17 with not otherwise specified eating disorders (NOS) (mean BMI-SDS: -1.4 ± 0.68), 30 with simple obesity (OB) (mean BMI-SDS: 6.91 ± 1.23) and 61 healthy (mean BMI-SDS: -0.18 ± 0.54).
Mean serum RES concentration in AN (2.8 ± 0.6 ng/ml) and NOS (3.1 ± 0.9 ng/ml) were significantly lower (p<0.0001) than in OB and H groups (4.8 ± 0.5 and 4.1 ± 0.4 ng/ml respectively). After corrected for BMI, RES values in AN were similar as in H subjects, but significantly higher (p<0.005) in comparison to OB group. ROC curve analysis revealed that 3.87 ng/ml is the threshold value of RES serum concentration differentiating AN from H girls (specificity 100%, sensitivity 80%). No significant correlations between BMI and serum resistin concentration are found in AN group, although a significant positive correlation has been established for all examined subjects.
Additional adaptive mechanisms may be involved in regulation of RES levels in adolescent girls with AN.
关于神经性厌食症（AN）患者血液抵抗素（RES）水平的研究较少，结果也很有限，但已证实这些患者脂肪组织中RES mRNA表达增加。本研究的目的是：1）评估AN女孩的血清抵抗素水平，并确定区分这些患者与健康受试者的阈值；2）分析受试对象血清抵抗素水平与BMI之间的关系。
使用ELISA试剂盒检测了195名青春期女孩的血清RES浓度：87名患有限制性AN（平均BMI-SDS：-2.65±0.2），17名患有未另行指定的进食障碍（NOS）（平均BMI-SDS：-1.4±0.68），30名患有单纯性肥胖（OB）（平均BMI-SDS：6.91±1.23），61名健康女孩（平均BMI-SDS：-0.18±0.54）。
AN组（2.8±0.6 ng/ml）和NOS组（3.1±0.9 ng/ml）的平均血清RES浓度显著低于OB组和健康组（H组）（分别为4.8±0.5和4.1±0.4 ng/ml，p<0.0001）。校正BMI后，AN组的RES值与H组受试者相似，但与OB组相比显著更高（p<0.005）。ROC曲线分析显示，3.87 ng/ml是区分AN与健康女孩的RES血清浓度阈值（特异性100%，敏感性80%）。AN组中未发现BMI与血清抵抗素浓度之间存在显著相关性，尽管在所有受试对象中已建立显著正相关。
患有AN的青春期女孩RES水平的调节可能涉及其他适应性机制。
