Kato Hisashi, Whiteside Theresa L
Department of Oral and Maxillofacial Surgery, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
Tokai J Exp Clin Med. 2011 Dec 20;36(4):144-51.
Interleukin-10 (IL-10) can mediate anti-tumor activity or tumor escape from the immune system. Here, we investigate IL-10 and IL-10recepter (IL-10R) expression in/on immune cells of patients with head and neck squamous cell carcinoma (HNSCC) and healthy volunteers as normal controls (NC) to further evaluate the dual role IL-10 might play in this disease.
Peripheral blood mononuclear cells from 15 HNSCC patients and 15 NC were stained and used for flow cytometry or stimulated first with OKT3/anti-CD28 Abs in the presence of IL-2 or with lipopolysaccharide and then stained for flow cytometry. The percentages of IL-10+ or IL-10R+ lymphocytes and monocytes and their mean fluorescence intensity were determined.
Monocytes had the highest frequency of IL-10+ and IL-10R+ cells before or after stimulation and the highest expression levels of these markers followed by CD8+ and then CD4+ T lymphocytes. No significant differences in the frequency or expression levels of IL-10 or IL-10R were observed between patients with HNSCC and NC.
Monocytes in the circulation of patients and NC are the main subset of IL-10- and IL-10R-expressing cells. The frequency of IL-10+ and IL-10R+ monocytes in patients with HNSCC is comparable to that in NC.
白细胞介素-10（IL-10）可介导抗肿瘤活性或使肿瘤逃避免疫系统。在此，我们研究头颈部鳞状细胞癌（HNSCC）患者和作为正常对照（NC）的健康志愿者免疫细胞中IL-10及其受体（IL-10R）的表达，以进一步评估IL-10在该疾病中可能发挥的双重作用。
对15例HNSCC患者和15例NC的外周血单个核细胞进行染色，用于流式细胞术检测；或先在有IL-2存在的情况下用OKT3/抗CD28抗体刺激，或用脂多糖刺激，然后进行染色用于流式细胞术检测。测定IL-10+或IL-10R+淋巴细胞和单核细胞的百分比及其平均荧光强度。
刺激前后，单核细胞中IL-10+和IL-10R+细胞的频率最高，这些标志物的表达水平也最高，其次是CD8+ T淋巴细胞，然后是CD4+ T淋巴细胞。HNSCC患者和NC之间在IL-10或IL-10R的频率或表达水平上未观察到显著差异。
患者和NC循环中的单核细胞是表达IL-10和IL-10R细胞的主要亚群。HNSCC患者中IL-10+和IL-10R+单核细胞的频率与NC中的相当。
