Medical School of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Clinics (Sao Paulo). 2012;67(6):587-90. doi: 10.6061/clinics/2012(06)07.
This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment.
Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured.
For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures.
Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.
本研究旨在评估手术治疗前后声门上型喉癌患者外周血单个核细胞刺激后产生的干扰素-γ和白细胞介素-10。
对 14 例晚期声门上型喉癌患者进行研究。在术前和术后晚期分离外周血单个核细胞进行培养，用刀豆蛋白 A 和卡介苗刺激，并测量上清液中干扰素-γ和白细胞介素-10 的浓度。
对于未刺激的培养物，术前和术后晚期培养物产生的干扰素-γ水平低于对照组培养物。刀豆蛋白 A 刺激后的干扰素-γ水平在术后晚期培养物中高于术前评估培养物。卡介苗刺激导致所有培养物产生相似水平的干扰素-γ和白细胞介素-10；因此，与相应的未刺激培养物相比，刺激增加了术前和术后培养物产生的干扰素-γ水平。
晚期声门上型喉癌患者的单核细胞体外产生干扰素-γ的能力存在缺陷。刺激后的细胞在术后似乎恢复了这种功能。
